S30 buffer solution | 20x stock | 500ml
- Product Code: GMES-B-S30
- Availability: In Stock
Tags: S30 buffer
S30 buffer solution | 20x stock
0.22μm filter-sterilised
For cell lysate generation and protein extraction
Not For dialysis use, dialysis use S30 is prepared at 5mM Tris-Acetate and 1mM DTT
Per 1000mL of 20x S30 buffer
|
Mixture A |
|
|
Tris-Acetate |
36.24 g (200mM, final concentration of 1X stock is 10mM) |
|
Magnesium acetate tetrahydrate |
60.00 g |
|
Potassium acetate |
117.80 g |
pH 8.2
Preparation
Dilute 1 volume of S30 (20X stock) with 19 volumes of Million-Q water to create a 1X stock.
Before use, add 1mL of 1M Dithiothreitol (DTT) to 500 mL of the 1X S30 buffer stock for a final concentration of 2mM. (DTT should be prepared separately by the user.)
Note
If precipitates appear at the bottom of the solution, heat the solution in a water bath at around 30~35°C and shake well.
After the water bath, use 70% alcohol to sterilise the outer surface of the bottle and cap before opening.
DO NOT use until the precipitate is completely dissolved.
*Because the solution is prone to form minor precipitates of Mg(OH)2/ MgCO3 at high pH, the original 50x stock product has been discontinued and updated to 20x stock.
