Lysine (LYS) medium | 250g
- Product Code: GMNB-LYS01
- Availability: In Stock
Tags: Lysine medium
Lysine (LYS) medium, 250g
Used for isolation and enumeration of wild yeasts in pitching yeasts.
Lysine medium contains, g/L
Dextrose, 44.5
Monopotassium phosphate, 1.78
Magnesium sulphate, 0.89
Calcium chloride, 0.178
Sodium chloride, 0.089
Adenine, 0.00178
DL-Methionine, 0.000891
L-Histidine, 0.000891
DL-Tryptophan, 0.000891
Boric acid, 0.0000089
Zinc sulphate, 0.0000356
Ammonium molybdate, 0.0000178
Manganese sulphate, 0.0000356
Ferrous sulphate, 0.0002225
L-Lysine, 1.00
Inositol, 0.02
Calcium pantothenate, 0.002
Aneurine, 0.0004
Pyridoxine, 0.0004
p-Aminobenzoic acid, 0.0002
Nicotinic acid, 0.0004
Riboflavin, 0.0002
Biotin, 0.000002
Folic acid, 0.000001
Agar, 17.80
Preparation
Suspend 6.6 g of Lysine Medium in 100 ml of distilled water containing 0.84 ml of potassium lactate solution ~60% (Cat. No. 60389). Boil to dissolve the medium completely. Cool to 50 °C. Adjust the pH to 5.0 with a 10% solution of Lactic Acid (Cat. No. 69778) and pour into sterile petri plates.
Note: The degree of contamination is expressed as the number of wild yeast cells per million cells of the original inoculum. The number of cells in the inoculum is important as a small number (100~1000) grow to a limited extent while 10,000 brewing yeast cells provide a direct measure of contaminant wild yeasts.
