Overview

Antifade Mounting Medium, Mowiol-Based, +/- DAPI - This Mowiol-based Antifade Mounting Medium is designed for fluorescence microscopy workflows requiring refractive-index matching, coverslip mounting, and protection against photobleaching.

The formulation is based on public-domain Mowiol-DABCO mountant chemistry and is offered with or without DAPI for nuclear counterstaining.

We also have

S30A Buffer · S30B Buffer · GMExpression Buffer catalogue 

Use and Applications

  • Immunofluorescence slide mounting
  • Fixed-cell and tissue-section microscopy
  • Routine imaging core facility workflows
  • IHC/IF Imaging Suite with antigen retrieval buffers and TBS/TBST

Specifications

ItemSpecification
Variant A10 mL dropper bottle, no DAPI
Variant B10 mL dropper bottle, DAPI 1 ug/mL
Bulk50 mL format for imaging cores
PackagingLight-protected bottle; DAPI variant protected from light

Composition 

ComponentAmount / concentrationFunction
Mowiol 4-88 / polyvinyl alcohol24% w/v; CAS 9002-89-5Mountant matrix
Glycerol60% v/v; CAS 56-81-5Refractive-index and viscosity control
Tris-HCl pH 8.5100 mMBuffering
DABCO2.5% w/v; CAS 280-57-9Antifade agent
DAPI1 ug/mL in optional variantNuclear counterstain

Instruction

1Bring mountant to room temperature and mix gently before use.
2Apply a small drop to the specimen and lower coverslip slowly to minimise bubbles.
3Allow curing according to the imaging protocol before sealing or long-term storage.
4Protect mounted slides from light.

Note

  • The DAPI-containing variant is convenient for standard fixed-cell imaging; choose the no-DAPI variant where nuclear staining is not desired or another channel is used.

Cautions

Caution. DAPI is a DNA-intercalating dye and a suspected mutagen. Avoid skin contact and ingestion, wear gloves, and dispose of DAPI-containing material as hazardous waste. The no-DAPI variant does not carry this hazard.
Caution. Not designed for live-cell imaging.

Storage and Expiry · Safety

  • Store at 2-8 deg C protected from light.
  • DAPI variant may be supplied frozen or refrigerated depending on final stability validation.
  • Transport. Ships at ambient temperature in insulated packaging (DAPI variant frozen/refrigerated and light-protected); place at the labelled storage temperature on receipt.
  • Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses. The mountant contains glycerol, Mowiol, Tris and DABCO (low hazard); the DAPI variant additionally requires mutagen-handling precautions and hazardous-waste disposal. Consult the Safety Data Sheet before use.

References

  1. Heimer and Taylor (1974), Journal of Clinical Pathology 27:254-256.
  2. Longin et al. (1993), Journal of Histochemistry & Cytochemistry 41:1833-1840.
  3. Safety Data Sheet

Frequently Asked Questions

Q1. Is this a hard-set mountant?
It is a liquid-curing Mowiol-based mountant. Curing behaviour should be validated against the customer's coverslip and imaging workflow.
Q2. Can DAPI concentration be customised?
Yes. DAPI-free, 1 ug/mL DAPI, and custom counterstain variants are feasible.