BSA Blocking Solution, 5%
AUD 125.00
Excl.GST
- Product Code: GMES-B-T4B14
- Availability: In Stock
Tags: Buffer
5% BSA Blocking Solution is a routine immunoassay reagent used to block non-specific binding sites on membranes, plates, and tissue sections.
Overview
BSA Blocking Solution, 5% - 5% BSA Blocking Solution is a routine immunoassay reagent used to block non-specific binding sites on membranes, plates, and tissue sections.
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Use and Applications
- Western blot membrane blocking
- ELISA plate blocking and antibody dilution
- IHC and IF blocking workflows
- SDS-PAGE Hand-Casting Suite and IHC/IF Imaging Suite companion product
Specifications
| Item | Specification |
|---|---|
| Bottle | 50 mL or 100 mL sterile bottle |
| Primary vehicle | TBS pH 7.6 with Tween 20 |
| Secondary vehicle | PBS variant available |
| Preservative | Azide-free by default |
Composition
| Component | Amount / concentration | Function |
|---|---|---|
| BSA Fraction V | 5% w/v; CAS 9048-46-8 | Blocking protein |
| Tris-HCl / NaCl | TBS vehicle | Buffer and ionic strength |
| Tween 20 | 0.05% optional | Background reduction |
| Ultrapure water | q.s. to final volume | Solvent |
Instruction
1Bring to room temperature and mix gently without foaming.
2Use directly for membrane or plate blocking according to the assay protocol.
3For antibody dilution, confirm the antibody datasheet supports BSA-containing diluent.
4Avoid returning used solution to the stock bottle.
Note
- Phospho-specific antibody workflows often prefer BSA over milk because milk contains phosphoproteins that can increase background.
Cautions
Caution. BSA solutions are microbial-growth-prone without azide. Use aseptic technique and respect the opened-bottle shelf life.
Caution. Contains bovine-derived protein; confirm institutional and import documentation requirements where relevant.
Storage and Expiry · Safety
- Store at 2-8 deg C.
- After opening, use within the labelled period; single-use 50 mL format recommended for low-throughput labs.
- Transport. Ships at ambient temperature in insulated packaging; brief transit temperature excursions are tolerated. Refrigerate at 2-8 deg C on receipt and protect from freezing.
- Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. Contains bovine-derived protein; handle per institutional biological-material and import requirements. Consult the Safety Data Sheet before use.
References
- Safety Data Sheet: SDS draft to be finalised before commercial release.
Frequently Asked Questions
Q1. Why azide-free?
Azide can interfere with HRP-based detection and creates hazardous waste concerns. Azide-free product is broader-use if handled aseptically.
