Sodium Citrate Antigen Retrieval Buffer, 10x stock, pH 6.0 | 500ml
AUD 46.00
Excl.GST
- Product Code: GMES-B-B11
- Availability: In Stock
Tags: Buffer
Sodium Citrate Antigen Retrieval Buffer is the standard acid-pH heat-induced epitope retrieval (HIER) buffer for formalin-fixed paraffin-embedded tissue sections.
Overview
Sodium Citrate Antigen Retrieval Buffer, pH 6.0, 10x - Sodium Citrate Antigen Retrieval Buffer is the standard acid-pH heat-induced epitope retrieval (HIER) buffer for formalin-fixed paraffin-embedded tissue sections.
The 10x stock format gives histology and imaging laboratories 5 L of 1x working buffer from one bottle while keeping shipping and storage volume low.
We also have
Tris-EDTA Antigen Retrieval · S30B Buffer · GMExpression Buffer catalogue
Use and Applications
- IHC antigen retrieval
- Immunofluorescence antigen retrieval
- Formalin-fixed tissue section processing
- IHC/IF Imaging Suite companion with Tris-EDTA retrieval buffer and antifade mountant
Specifications
| Item | Specification |
|---|---|
| Bottle | 500 mL 10x stock, makes 5 L working buffer |
| pH | pH 6.0 after dilution |
| Surfactant | Tween 20 included |
| Sterility | 0.22 μm Filtered for clarity and microbial control |
Composition
| Component | Amount / concentration | Function |
|---|---|---|
| Trisodium citrate dihydrate | 100 mM in 10x; CAS 6132-04-3 | Citrate buffer |
| Tween 20 | 0.05% in 10x | Wetting and retrieval consistency |
| HCl | q.s. to pH 6.0 | pH adjustment |
| Ultrapure water | q.s. to final volume | Solvent |
Instruction
1Dilute 1 volume of 10x stock with 9 volumes of ultrapure water.
2Heat slides in diluted buffer according to the tissue and antibody protocol.
3Allow controlled cooling before proceeding to blocking and antibody incubation.
4Discard used retrieval buffer after tissue processing.
Note
- Citrate pH 6.0 and Tris-EDTA pH 9.0 retrieval conditions are complementary; antibody datasheets often specify which is preferred.
Cautions
Caution. Use heat-resistant staining vessels and follow microwave, pressure-cooker, or water-bath safety procedures.
Caution. Do not mix concentrated retrieval buffer directly with tissue sections.
Storage and Expiry · Safety
- Store at room temperature or 2-8 deg C.
- Unopened shelf life target: 24 months after validation.
- Transport. Ships at ambient temperature; no cold chain required. Protect from freezing and from temperatures above 40 deg C.
- Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. During heat-mediated antigen retrieval the buffer reaches near-boiling temperature; use heat-resistant vessels, insulated gloves, and a face shield to prevent scald/steam burns. Consult the Safety Data Sheet before use.
References
- Safety Data Sheet
Frequently Asked Questions
Q1. When should citrate pH 6.0 be used?
Use it when the antibody or tissue protocol specifies acid-pH HIER, or when empirical optimisation shows stronger signal and lower background than alkaline retrieval.
Q2. Can it be paired with fluorescence workflows?
Yes. It is suitable for IF as well as chromogenic IHC when validated for the antibody.
