ATCC medium 2693, Modified Dixon medium, mDixon, without Agar I 217g + 33ml
- Product Code: GMNB-MDM02
- Availability: 2-3 weeks
Tags: mDixon-wo
Modified Dixon medium (mDixon | without agar)
ATCC medium: 2693 | for fungal culturing
Bottle A (Powder) : 217 g
Bottle B (Tween 40) : 33ml (~35g)
Per 1000mL of mDixon medium contains
Malt Extract 36 g
Desiccated Oxbile 20 g
Peptone 6 g
Glycerol 2 ml
Oleic Acid 2 ml
Tween 40 10 ml
Preparation
Weigh 66.0 g powder from bottle A, take 10 ml Tween 40 (bottle B) [1], heat and dissolve well in 800 ml distilled water or MQ water, then make up to 1000 ml, aliquot if necessary. Autoclave at 121°C for 15min.
[1] Before adding Tween 40, it is suggested to be warmed in a water bath at ~ 50°C for 10 minutes, to reduce the viscosity and make it easy to operate.
Note: This medium contains much oleic acid, and there will be a small amount of flocculent precipitate after sterilisation.
Safety Data Sheet:
