• S30B buffer solution, 10x stock, 20x stock | 500ml

S30B buffer solution, 10x stock, 20x stock | 500ml

AUD 125.00    Excl.GST
  • Product Code: GMES-B-S30B
  • Availability: In Stock

Tags: S30B buffer

S30B buffer solution | 10x stock

S30B buffer solution | 20x stock

0.22μm filter-sterilised 


For cell lysate generation and protein extraction

S30A/B provides additional glutamate compared to S30, which can support the tricarboxylic acid cycle to produce more energy molecules (e.g. ATP) and provide a longer active window for cell lysates.


Per 1000mL of S30B buffer

Tris Base                 0.606g

Mg-Glutamate 5.44g

K-Glutamate         12.194g

pH   8.2


Preparation

Dissolve 1 volume of  S30B buffer 10x stock in 9 volumes of million-Q water, and use directly.

Or Dissolve 1 volume of  S30B buffer 20X stock in 19 volumes of million-Q water, and use directly.

Before use add 1mL of 1 M DTT(2mM, final concentration) to 500mL S30B buffer.


Please contact us if a customised HEPES version of S30B Buffer is needed.


*Heat the solution in a water bath to over 30°C if precipitates appear at the bottom of the solution. DO NOT use until the precipitate is completely dissolved.

*Because the solution is prone to form minor precipitates of Mg(OH)/ MgCO3 at high pH, the original 50x stock product has been discontinued and updated to 10x stock.

Safety Data Sheet:

SDS_S30B_10X_en
SDS_S30B_20X_en