S30B buffer solution, 10x stock, 20x stock | 500ml
- Product Code: GMES-B-S30B
- Availability: In Stock
Tags: S30B buffer
S30B buffer solution | 10x stock
S30B buffer solution | 20x stock
0.22μm filter-sterilised
For cell lysate generation and protein extraction
S30A/B provides additional glutamate compared to S30, which can support the tricarboxylic acid cycle to produce more energy molecules (e.g. ATP) and provide a longer active window for cell lysates.
Per 1000mL of S30B buffer
Tris Base 0.606g
Mg-Glutamate 5.44g
K-Glutamate 12.194g
pH 8.2
Preparation
Dissolve 1 volume of S30B buffer 10x stock in 9 volumes of million-Q water, and use directly.
Or Dissolve 1 volume of S30B buffer 20X stock in 19 volumes of million-Q water, and use directly.
Before use add 1mL of 1 M DTT(2mM, final concentration) to 500mL S30B buffer.
Please contact us if a customised HEPES version of S30B Buffer is needed.
*Heat the solution in a water bath to over 30°C if precipitates appear at the bottom of the solution. DO NOT use until the precipitate is completely dissolved.
*Because the solution is prone to form minor precipitates of Mg(OH)2 / MgCO3 at high pH, the original 50x stock product has been discontinued and updated to 10x stock.
