SDS-PAGE Stacking Gel Buffer, 1M Tris-HCl, pH 6.8 | 500ml
AUD 40.00
Excl.GST
- Product Code: GMES-B-B4
- Availability: In Stock
Tags: Buffer
Stacking Gel Buffer is the pH 6.8 Tris-HCl component of the classical Laemmli SDS-PAGE system. It creates the discontinuous pH environment needed to concentrate proteins into a sharp band before entry into the resolving gel.
Overview
SDS-PAGE Stacking Gel Buffer, 1 M Tris-HCl, pH 6.8 - Stacking Gel Buffer is the pH 6.8 Tris-HCl component of the classical Laemmli SDS-PAGE system. It creates the discontinuous pH environment needed to concentrate proteins into a sharp band before entry into the resolving gel.
The SKU is labelled for gel casting to reduce the common error of swapping stacking and resolving gel buffers.
We also have
SDS-PAGE Resolving Gel Buffer · S30B Buffer · GMExpression Buffer catalogue ·
Use and Applications
- Hand-casting SDS-PAGE stacking gels
- Protein electrophoresis teaching and core-facility workflows
- SDS-PAGE Hand-Casting Suite with resolving gel buffer and Laemmli sample buffer
- Routine Western blot sample preparation workflows
Specifications
| Item | Specification |
|---|---|
| Bottle | 500 mL sterile bottle |
| Concentration | 1 M Tris-HCl, pH 6.8 |
| Companion | SDS-PAGE Resolving Gel Buffer pH 8.8 |
| Label note | Application-specific SDS-PAGE gel-casting instructions |
Composition
| Component | Amount / concentration | Function |
|---|---|---|
| Tris base | Calculated ratio for pH 6.8 | Base form |
| Tris-HCl | Calculated ratio for pH 6.8 | Acid form |
| MQ water | q.s. to final volume | Solvent |
Instruction
1Use as the Tris component in stacking gel recipes.
2Mix with acrylamide/bis solution, SDS, water, APS, and TEMED according to the protocol.
3Overlay on the polymerised resolving gel and insert comb immediately.
Note
- This is also the base pH for many Laemmli sample buffer formulations.
Cautions
Caution. Do not substitute resolving gel buffer for stacking gel buffer; pH mismatch changes stacking behaviour.
Caution. Acrylamide handling requires separate institutional safety controls.
Storage and Expiry · Safety
- Store at room temperature or 2-8 deg C.
- Unopened shelf life: 24 months from manufacture after QC validation.
- Transport. Ships at ambient temperature; no cold chain required. Protect from freezing and from temperatures above 40 deg C.
- Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. This buffer is Tris-HCl only (SDS is supplied separately in the running and sample buffers); Tris is a mild irritant, so avoid eye contact. Consult the Safety Data Sheet before use.
References
- Laemmli (1970), Nature 227:680-685.
Frequently Asked Questions
Q1. Does this replace Laemmli sample buffer?
No. It is a gel-casting buffer. Laemmli sample buffer contains SDS, glycerol, tracking dye, and optional reducing agent.
Q2. Can it be used as generic Tris-HCl pH 6.8?
Yes, but it is labelled and documented for stacking gel preparation.
