Gram-negative bacteria (G-) selective medium | 250g
- Product Code: GMNB-GNSM01
- Availability: In Stock
Tags: G- selective medium
Gram-negative bacteria (G-) selective medium
Usage
For the selective culture of Gram-negative bacteria.
Characteristics of bacterial growth on G- selective medium:
Inoculate with the following quality control strains and place in 36±1°C aerobic culture for 18-24 hours.
QC strain Strain No. Inoculum amount Growth status Description
Escherichia coli ATCC25922 / +++ Good Growth
Salmonella ATCC14028 / +++ Good Growth
Staphylococcus aureus ATCC25923 / - Inhibited
Per 1000mL of G- selective medium contains
Peptone 5 g
Yeast extract 2 g
Crystalline Violet 0.002 g
Skimmed Milk Powder 1 g
Streptococcus lactis peptide(Nisin) 0.0016 g
Agar 15 g
pH 7.4 ± 0.2 at 25°C
Preparation
Weigh 23.0g of this product, dissolve in 1000ml distilled water with heating and stirring, divide into triangular flasks, 200ml per bottle, autoclave at 121°C for 15 minutes, when it cools down to 45-50°C, add 1 piece of sterile penicillin solution (containing penicillin G 96 units) to each bottle, mix well and pour it into the sterile petri dish.
