Overview

MOPS Buffer, 1M, pH 7.0 - MOPS buffers the neutral pH band occupied by many RNA, protein, and organelle workflows while avoiding phosphate-driven metal precipitation.

The product is offered as a 1 M stock for general buffering and can be extended to a MOPS RNA Running Buffer 10x variant for formaldehyde RNA agarose gel workflows.

We also have

S30A Buffer · S30B Buffer · GMExpression Buffer catalogue · YCFA Modified Medium · Modified Chopped Meat Broth

Use and Applications

  • Denaturing RNA agarose gel running buffer preparation
  • NuPAGE-style Bis-Tris protein gel running systems
  • Mitochondrial isolation and phosphate-sensitive cell biology
  • Neutral pH enzyme assays where Tris or phosphate are unsuitable

→Buffer Indicator Navigator

Use the GMExpression Buffer Indicator Navigator to find suitable pH ranges that match this buffer system, or start from a specific buffer and search for suitable working pH ranges for the intended experiment.

Specifications

ItemSpecification
Bottle500 mL amber borosilicate bottle
Stock1 M MOPS, pH 7.0
VariantMOPS RNA Running Buffer 10x available as companion SKU
Sterility0.2 um sterile-filtered

Composition 

ComponentAmount / concentrationFunction
MOPS free acid1 M; CAS 1132-61-2Primary buffering compound
NaOHq.s. to pH 7.0pH adjustment
Ultrapure waterq.s. to final volumeRNase-controlled solvent where required
RNA running variant0.2 M MOPS, 0.05 M sodium acetate, 0.01 M EDTA in 10x stockReady formulation for RNA gels

Instruction

1Dilute the 1 M stock to the required working concentration.
2For RNA gel buffer, use the dedicated MOPS RNA Running Buffer 10x variant and dilute 1:10 with RNase-controlled water.
3Protect MOPS-containing solutions from unnecessary heat and light exposure.
4Do not autoclave; sterile filtration is the preferred manufacturing and user-side sterilisation route.

Note

  • MOPS may develop a yellow-brown colour if overheated or exposed to light for long periods. Use amber packaging for stock storage.

Cautions

Caution. Do not autoclave MOPS stocks for RNA work; heat can generate UV-active degradation products.
Caution. Confirm pH at the working temperature for temperature-sensitive assays.

Storage and Expiry · Safety

  • Store at 2-8 deg C in amber bottle.
  • Unopened shelf life: 12 months from manufacture after QC validation.
  • Transport. Ships at ambient temperature in insulated packaging; brief transit temperature excursions are tolerated. Refrigerate at 2-8 deg C on receipt and protect from freezing.
  • Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. The formulated buffer presents no significant hazard under normal use; avoid ingestion and eye contact. Consult the Safety Data Sheet before use.

References

  1. Good et al. (1966), Biochemistry 5:467-477.
  2. Sambrook and Russell, Molecular Cloning, 3rd edition.

Frequently Asked Questions

Q1. Is MOPS interchangeable with HEPES?
Often no. Both buffer near neutral pH, but MOPS is preferred for RNA gel and some Bis-Tris protein-gel systems.
Q2. Why offer a 10x RNA running variant?
It prevents repeated in-lab weighing of MOPS, sodium acetate, and EDTA for a routine RNA electrophoresis workflow.