Overview

Cas9 / Cas12 RNP Electroporation Buffer - Cas9/Cas12 RNP Electroporation Buffer is a high-value specialty buffer for genome-editing workflows where ionic strength, pH, magnesium concentration, and osmotic compatibility must be tightly controlled.

The product is positioned for customers who need a standalone buffer rather than a bundled proprietary electroporation kit, especially in pharma R&D and platform genome-editing laboratories.

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S30A Buffer · S30B Buffer · GMExpression Buffer catalogue · YCFA Modified Medium · Modified Chopped Meat Broth

Use and Applications

  • Cas9 and Cas12 RNP handling before electroporation
  • Mammalian cell electroporation protocol development
  • Genome-editing assay optimisation in pharma and biotech R&D
  • Small-batch custom buffer development for cell-line-specific electroporation settings

→Buffer Indicator Navigator

Use the GMExpression Buffer Indicator Navigator to find suitable pH ranges that match this buffer system, or start from a specific buffer and search for suitable working pH ranges for the intended experiment.

Specifications

ItemSpecification
Pack5 x 1 mL aliquots, filled aseptically from 0.1 um (mycoplasma-reduction grade) filtered bulk
Buffer systemHEPES-KOH pH 7.5 with acetate salts
QualityDEPC-water-grade preparation option
CustomisationSE/SF-style ionic-strength variants available after technical review

Composition 

ComponentAmount / concentrationFunction
HEPES-KOHpH 7.5Primary pH buffer
Potassium acetateCell-compatible K+ ionic strengthElectroporation conductivity control
Magnesium acetateLow millimolar Mg2+RNP and membrane compatibility
GlycerolStabilising excipientRNP handling stability
Nuclease-controlled waterq.s. to final volumeSolvent

Instruction

1Thaw one aliquot on ice immediately before use.
2Mix RNP gently with the buffer according to the electroporation protocol.
3Avoid prolonged room-temperature exposure before pulse delivery.
4Discard unused aliquot after thawing unless the workflow has validated refreezing.

Note

  • Electroporation conditions are cell-line dependent. This buffer is a controlled starting formulation, not a universal replacement for all proprietary nucleofection solutions.

Cautions

Caution. Use nuclease-free technique for RNP workflows.
Caution. Validate cell viability and editing efficiency for each cell type and electroporation device.

Sterile Filtration - Mycoplasma-Reduction Grade

Because edited cells are returned to mammalian culture, the bulk buffer is sterilised through a 0.1 um membrane rather than the conventional 0.22 um sterilising grade. Mycoplasma lack a cell wall and are deformable enough to pass through 0.22 um pores; a 0.1 um membrane gives a substantially higher mycoplasma-retention margin, and each lot is released mycoplasma-test-negative by PCR. This protects the downstream culture as well as the editing reaction.

Storage and Expiry · Safety

  • Store at -20 deg C or -80 deg C according to released stability data.
  • Ship frozen.
  • Transport. Ships frozen on dry ice or gel ice; transfer to the stated storage temperature immediately on receipt and minimise freeze-thaw cycles.
  • Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. The buffer presents no significant chemical hazard; use nuclease-free, aseptic technique. Consult the Safety Data Sheet before use.

References

  1. Supplier landscape reviewed in the strategic expansion report: IDT, Lonza, Synthego RNP delivery systems.
  2. Safety Data Sheet

Frequently Asked Questions

Q1. Is this a transfection reagent?
No. It is a buffer for RNP handling and electroporation setup. The customer supplies RNP and electroporation hardware.
Q2. Can it be made cell-line specific?
Yes. The candidate is suitable for custom ionic-strength variants after pilot testing.