Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium) 50x stock | For 25L
- Product Code: GMNB-BMFN01
- Availability: Pre-Order
Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium; Modified BBM)
500ml, 50x stock, for preparing 25L of liquid medium
Filter-sterilised 0.22µm.
Bold Basal Medium Modified with trace elements and replaced MoO3 with Sodium Molybdate.
Applications
Modified Bold Basal Medium is an axenic-culture maintenance medium in phycology/algae studies.
Commonly used for the cultivation of a variety of freshwater algae cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). Other algae species like Zygnemataceae (Zygogonium ericetorum) can also be grown in Bold’s Basal Medium(Stancheva et al., 2014)
Components, mg per litre
Macro Elements |
|
|
NaNO3 |
250 |
|
K2HPO4 |
75 |
|
KH2PO4 |
175 |
|
MgSO4 ·7H2O |
75 |
|
CaCl2 · 2H2O |
25 |
|
NaCl |
25 |
|
KOH |
31 |
|
Na2 · EDTA |
63.61 |
Micro Elements |
|
|
H3BO3 |
11.42 |
|
ZnSO4 ·7H2O |
8.82 |
|
FeSO4 ·7H2O |
4.98 |
|
Co(NO3)2 ·6H2O |
0.49 |
|
CuSO4 · 5H2O |
1.57 |
|
MnCl2 · 4H2O |
1.44 |
|
Na2MoO4 |
1.19 |
|
Trace Elements |
|
|
NiCl2 · 6H2O |
0.003 |
|
Potassium iodide |
0.003 |
|
Sodium selenite |
0.002 |
|
Stannic chloride |
0.001 |
|
Vanadium sulphate |
0.0022 |
Preparation
Dissolve 1 volume of the media solution 50x stock in 49 volumes of million-Q water, add 1 mL of 0.1% Sulphuric Acid solution to 1 litre of Bold Basal Media liquid.
The final solution pH should be adjusted to 6.6 +/- 0.1 with 5M KOH and Sulphuric Acid.
Storage
When the liquid media is prepared, please store it at 2~8°C, the shelf life of such a stock can be several years as all ingredients and elements are in a stable inorganic salt form.
Safety Data Sheet:
Instructions:
