Woody Plant Medium, WPM medium | Without Sucrose & Agar | 250g
- Product Code: GMNB-WPM02
- Availability: In Stock
Tags: Woody Plant Medium, WPM medium| Without Sucrose & Agar | 250g
Woody Plant Medium, WPM medium, Lloyd & McCown Woody Plant Basal Medium | With Sucrose & Agar
250g, fine powder
Lloyd and McCown formulated Woody Plant Medium in 1981 for culturing Mountain Laurel (Kalmia latifolia) shoot tips.
Woody Plant Medium (WPM) contains all the necessary macro and microelements. Potassium dihydrogen phosphate is used as a phosphate source, while calcium is also present. Nitrate provides nitrogen to the plant. Microelements such as Boron, Manganese, Iron, Molybdenum, and Zinc play a vital role in plant metabolism. Boron plays a key role in carbohydrate metabolism. Thiamine, pyridoxine, and nicotinic acid act as enzymatic cofactors in universal metabolism. Plants have pathways for glycolysis and the TCA cycle, which are involved in primary and secondary metabolism. Glycine is used as an amino acid source.
Applications
WPM is commonly used to propagate various woody plant species.
Plant tissue culture media for shoot regeneration, and root formation.
Components, mg per litre
|
Micro Elements |
|
|
H3BO3 |
6.2 |
|
MnSO4.H2O |
22.3 |
|
ZnSO4.7H2O |
8.6 |
|
Na2MoO4.2H2O |
0.25 |
|
CuSO4.5H2O |
0.25 |
|
EDTA·2Na |
37.3 |
|
FeSO4 |
27.8 |
|
Macro Elements |
|
|
KNO3 |
990 |
|
Ca(NO3)2.4H2O |
556 |
|
NH4NO3 |
400 |
|
KH2PO4 |
170 |
|
MgSO4.7H2O |
370 |
|
CaCl2.2H2O |
96 |
|
Vitamins |
|
|
Myo-inositol |
100.0 |
|
Glycine |
2.0 |
|
Thiamine HCl |
1.0 |
|
Pyridoxine HCl |
0.5 |
|
Nicotinic acid |
0.5 |
Above is the universal formula. Adjust Ingredients according to any specific protocols if needed.
Preparation
Suspend 2.78 g of the medium into distilled water. Mix well and make up to 1000ml. Heat with continuous stirring and aliquot into tubes or flasks. Sterilise in the autoclave at 121°C for 15 minutes.
^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances vigorously.
Storage
Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant.
