Knudson C Orchid Medium with morel modification | WO Vitamins & Sucrose | 100L

This item is not usually in stock due to low daily demand and long-term storage challenges on raw materials.

Upon receiving your order, we will commence with the manufacturing process. The processing time will be approximately 4 to 6 weeks.

Knudson C Orchid Medium with morel modification | WO Vitamins & Sucrose

Fine powder, applied for 100L media preparation

Applications

For use in plant cell culture and micropropagation.

Knudson C Orchid Medium with morel modification consists of the macroelements and microelements as described by Knudson (1946) and Morel (1965). The medium was originally developed for the in vitro germination of Cymbidium orchid seeds but can also be used for other orchid species.

Components, mg per litre

Micro Elements         

FeSO4.7H2O                   25.00

MnSO4.H2O                   5.68

Macro Elements        

Ca(NO3)2                    241.30

KCl                                    250.00

KH2PO4                            250.00

MgSO4                            122.15

NH4NO3                     500.00

(NH4)2SO4                     500.00

The total concentration of Micro and Macro elements including vitamins: 1894mg/l

Vitamins（optional）*      

Auxins（optional）**

Sucrose & Agar （optional）**

Sucrose (as carbohydrate)        20,000

Agar                      A.C.D.

Charcoal                   A.C.D.  (suggest 2,000)

Banana Powder         A.C.D.  (suggest 25,000) (100,000 for fresh banana)

A.C.D.~ As Customers' Demands

Preparation

Suspend 1.89g of the Knudson C Orchid Medium into distilled water^, Mix well and make up to 1000ml. Heat with continuous stirring,  Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Sterilise in the autoclave at 121℃ for 15 minutes (the Knudson C Orchid Medium with Sucrose should be autoclaved at /115℃ for 20 minutes or might turn dark at over 115℃).