Knudson C Orchid Medium with morel modification | WO Vitamins & Sucrose | 100L
- Product Code: GMNB-KNDC-M01
- Availability: 4-6 weeks
Tags: Knudson C Medium
This item is not usually in stock due to low daily demand and long-term storage challenges on raw materials.
Upon receiving your order, we will commence with the manufacturing process. The processing time will be approximately 4 to 6 weeks.
Knudson C Orchid Medium with morel modification | WO Vitamins & Sucrose
Fine powder, applied for 100L media preparation
Applications
For use in plant cell culture and micropropagation.
Knudson C Orchid Medium with morel modification consists of the macroelements and microelements as described by Knudson (1946) and Morel (1965). The medium was originally developed for the in vitro germination of Cymbidium orchid seeds but can also be used for other orchid species.
Components, mg per litre
Micro Elements
FeSO4.7H2O 25.00
MnSO4.H2O 5.68
Macro Elements
Ca(NO3)2 241.30
KCl 250.00
KH2PO4 250.00
MgSO4 122.15
NH4NO3 500.00
(NH4)2SO4 500.00
The total concentration of Micro and Macro elements including vitamins: 1894mg/l
Vitamins(optional)*
Auxins(optional)**
Sucrose & Agar (optional)**
Sucrose (as carbohydrate) 20,000
Agar A.C.D.
Charcoal A.C.D. (suggest 2,000)
Banana Powder A.C.D. (suggest 25,000) (100,000 for fresh banana)
A.C.D.~ As Customers' Demands
Preparation
Suspend 1.89g of the Knudson C Orchid Medium into distilled water^, Mix well and make up to 1000ml. Heat with continuous stirring, Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Sterilise in the autoclave at 121℃ for 15 minutes (the Knudson C Orchid Medium with Sucrose should be autoclaved at /115℃ for 20 minutes or might turn dark at over 115℃).