Murashige and Skoog medium, with Agar and Sucrose | 250g
- Product Code: GMNB-MSM125
- Availability: In Stock
Murashige and Skoog medium, with Agar and Sucrose
250g, fine powder
Murashige and Skoog medium (MS, MSO/MS0,
MS Basal media) is a plant growth medium used for the
plant cell culture.
Applications
The principal component of In-vitro plant
cultivation.
Plant tissue culture media like calli induction,
shoot regeneration, and root formation.
For the germination of seeds of different plant
species like Arabidopsis thaliana, Nicotiana benthamiana, under sterile growth
conditions.
Components, mg per
litre
|
Micro Elements |
|
|
KI |
0.83 |
|
H3BO3 |
6.2 |
|
MnSO4.H2O |
22.3 |
|
ZnSO4.7H2O |
8.6 |
|
Na2MoO4.2H2O |
0.25 |
|
CoCl2.6H2O |
0.025 |
|
CuSO4.5H2O |
0.025 |
|
EDTA·2Na |
37.3 |
|
FeSO4 |
27.8 |
|
Macro Elements |
|
|
KNO3 |
1900 |
|
NH4NO3 |
1650 |
|
KH2PO4 |
170 |
|
MgSO4.7H2O |
370 |
|
CaCl2.2H2O |
440 |
|
Vitamins |
|
|
Inositol |
100.0 |
|
Glycine |
2.0 |
|
Thiamine HCl |
0.1 |
|
Pyridoxine HCl |
0.5 |
|
Nicotinic acid |
0.5 |
|
Sucrose & Agar |
|
|
Sucrose |
30.0 |
|
Agar |
7.0 |
Final pH 5.7±0.1 at 25°C
Above is the universal formula. Adjust Ingredients according to
any specific protocols if needed.
Preparation
Suspend 41.7
g of the medium into distilled water. Mix well and make up to 1000ml.
Heat with continuous stirring and aliquot into tubes or flasks. Sterilise in the autoclave at 115°C for 30 minutes (the media
with Sucrose may turn dark at over 115°C).
^ During preparation, avoid naked flames and do not mix the
basal salt mixture with reducing substances vigorously.
Storage
Media powder is extremely hygroscopic and should be protected from
atmospheric moisture. If possible, the unused powder should be stored in a
sealed box with silica desiccant.
Due to Australian Customs restrictions for flammable & volatile chemicals, it is strongly recommended that international buyers email us to confirm shipping availability prior to purchase.
