• Woody Plant Medium, WPM medium | With Sucrose & Agar | 250g

Woody Plant Medium, WPM medium | With Sucrose & Agar | 250g

AUD 69.00    Excl.GST
  • Product Code: GMNB-WPM01
  • Availability: In Stock

Tags: Woody Plant Medium, WPM medium| Without Sucrose & Agar | 250g

Woody Plant Medium, WPM medium, Lloyd & McCown Woody Plant Basal Medium | With Sucrose & Agar 

250g, fine powder


Lloyd and McCown formulated Woody Plant Medium in 1981 for culturing Mountain Laurel (Kalmia latifolia) shoot tips. 


Woody Plant Medium (WPM) contains all the necessary macro and microelements. Potassium dihydrogen phosphate is used as a phosphate source, while calcium is also present. Nitrate provides nitrogen to the plant. Microelements such as Boron, Manganese, Iron, Molybdenum, and Zinc play a vital role in plant metabolism. Boron plays a key role in carbohydrate metabolism. Thiamine, pyridoxine, and nicotinic acid act as enzymatic cofactors in universal metabolism. Plants have pathways for glycolysis and the TCA cycle, which are involved in primary and secondary metabolism. Glycine is used as an amino acid source.


Applications

WPM is commonly used to propagate various woody plant species.  

Plant tissue culture media for shoot regeneration, and root formation.


Components, mg per litre

Micro Elements

 

H3BO3

6.2

MnSO4.H2O

22.3

ZnSO4.7H2O

8.6

Na2MoO4.2H2O

0.25

CuSO4.5H2O

0.25

EDTA·2Na

37.3

FeSO4

27.8

 

Macro Elements

 

KNO3

990

Ca(NO3)2.4H2O

556

NH4NO3

400

KH2PO4

170

MgSO4.7H2O

370

CaCl2.2H2O

96

 

Vitamins

 

Myo-inositol

100.0

Glycine

2.0

Thiamine HCl

1.0

Pyridoxine HCl

0.5

Nicotinic acid

0.5

 

Sucrose & Agar

 

Sucrose

20000

Agar - (High strength)

7000

 

Above is the universal formula. Adjust Ingredients according to any specific protocols if needed.

 

Preparation

Suspend 29.78 g of the medium into distilled water. Mix well and make up to 1000ml. Heat with continuous stirring and aliquot into tubes or flasks. Sterilise in the autoclave at 115°C for 30 minutes (the media with Sucrose may turn dark at over 115°C).

^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances vigorously.

 

Storage

Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant.