1/2 Murashige and Skoog medium, 1/2 MS medium | Without Agar and Sucrose | 250g

1/2 Murashige and Skoog medium

250g, fine powder

1/2 Murashige and Skoog medium (1/2 MS or Half-strength MS Basal media) is a plant growth medium used for the plant cell culture.

The 1/2 MS medium has macroelements at half strength, but microelements and vitamins at full strength, as described by MURASHIGE & SKOOG (1962).

This modification is often used for the cultivation of plant species that are sensitive to high salt concentrations, and commonly used for plant cuttings and rooting.

Applications

The principal component of In-vitro plant cultivation.

Plant tissue culture media like calli induction, shoot regeneration, and root formation.

For the germination of seeds of different plant species like Arabidopsis thaliana, Nicotiana benthamiana, under sterile growth conditions.

Components, mg per litre

Above is the universal formula. Adjust Ingredients according to any specific protocols if needed.

Preparation

Suspend 2.47 g of the medium into distilled water. Mix well and make up to 1000ml. Heat with continuous stirring and aliquot into tubes or flasks. Sterilise in the autoclave at 121°C for 15 minutes.

^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances vigorously.

Storage

Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant.

Due to Australian Customs restrictions for flammable & volatile chemicals, it is strongly recommended that international buyers email us to confirm shipping availability prior to purchase.