1/2 Murashige and Skoog medium, 1/2 MS medium | With Agar and Sucrose| 250g
- Product Code: GMNB-MSM120
- Availability: 2-3 weeks
Tags: 1/2 MS medium
1/2 Murashige and Skoog medium
250g, fine powder
1/2 Murashige and Skoog medium (1/2 MS or Half-strength MS Basal media) is
a plant growth medium used for the plant cell culture.
The 1/2 MS medium has macroelements at half
strength, but microelements and vitamins at full strength, as described by
MURASHIGE & SKOOG (1962).
This modification is often used for the cultivation
of plant species that are sensitive to high salt concentrations, and commonly
used for plant cuttings and rooting.
Applications
The principal component of In-vitro plant
cultivation.
Plant tissue culture media like calli induction,
shoot regeneration, and root formation.
For the germination of seeds of different plant
species like Arabidopsis thaliana, Nicotiana benthamiana, under sterile growth
conditions.
Components, mg per
litre
Micro Elements |
|
KI |
0.83 |
H3BO3 |
6.2 |
MnSO4.H2O |
22.3 |
ZnSO4.7H2O |
8.6 |
Na2MoO4.2H2O |
0.25 |
CoCl2.6H2O |
0.025 |
CuSO4.5H2O |
0.025 |
EDTA·2Na |
37.3 |
FeSO4 |
27.8 |
Macro Elements |
|
KNO3 |
950 |
NH4NO3 |
825 |
KH2PO4 |
85 |
MgSO4.7H2O |
185 |
CaCl2.2H2O |
220 |
Vitamins |
|
Inositol |
100.0 |
Glycine |
2.0 |
Thiamine HCl |
0.1 |
Pyridoxine HCl |
0.5 |
Nicotinic acid |
0.5 |
Sucrose & Agar |
|
Sucrose |
30000 |
Agar - (High strength) |
7000 |
Above is the universal formula. Adjust Ingredients according to
any specific protocols if needed.
Preparation
Suspend 39.47 g of the medium
into distilled water. Mix well and make up to 1000ml. Heat with continuous stirring and
aliquot into tubes or flasks. Sterilise in the autoclave at 115°C for 30
minutes (the media with Sucrose may turn dark at over 115°C).
^ During preparation, avoid naked flames and do not mix the
basal salt mixture with reducing substances vigorously.
Storage
Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant.