1/2 Murashige and Skoog medium, 1/2 MS medium | With Agar and Sucrose| 250g

AUD 69.00 Excl.GST

Product Code: GMNB-MSM120
Availability: 2-3 weeks

Qty

Description

1/2 Murashige and Skoog medium

250g, fine powder

1/2 Murashige and Skoog medium (1/2 MS or Half-strength MS Basal media) is a plant growth medium used for the plant cell culture.

The 1/2 MS medium has macroelements at half strength, but microelements and vitamins at full strength, as described by MURASHIGE & SKOOG (1962).

This modification is often used for the cultivation of plant species that are sensitive to high salt concentrations, and commonly used for plant cuttings and rooting.

Applications

The principal component of In-vitro plant cultivation.

Plant tissue culture media like calli induction, shoot regeneration, and root formation.

For the germination of seeds of different plant species like Arabidopsis thaliana, Nicotiana benthamiana, under sterile growth conditions.

Components, mg per litre

Micro Elements
KI	0.83
H₃BO₃	6.2
MnSO₄.H₂O	22.3
ZnSO₄.7H₂O	8.6
Na₂MoO₄.2H₂O	0.25
CoCl₂.6H₂O	0.025
CuSO₄.5H₂O	0.025
EDTA·2Na	37.3
FeSO₄	27.8

Macro Elements
KNO₃	950
NH₄NO₃	825
KH₂PO₄	85
MgSO₄.7H₂O	185
CaCl₂.2H₂O	220

Vitamins
Inositol	100.0
Glycine	2.0
Thiamine HCl	0.1
Pyridoxine HCl	0.5
Nicotinic acid	0.5

Sucrose & Agar
Sucrose	30000
Agar - (High strength)	7000

Above is the universal formula. Adjust Ingredients according to any specific protocols if needed.

Preparation

Suspend 39.47 g of the medium into distilled water. Mix well and make up to 1000ml. Heat with continuous stirring and aliquot into tubes or flasks. Sterilise in the autoclave at 115°C for 30 minutes (the media with Sucrose may turn dark at over 115°C).

^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances vigorously.

Storage

Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant.