Gamborg B5 medium | W / WO Vitamins | 50/100L
- Product Code: GMNB-GB5M01
- Availability: In Stock
Tags: Gamborg B5 medium
Gamborg B5 medium |
W/WO Vitamins
-Gamborg B5 Basal Salt
Mixture, W/O Vitamins
-Gamborg B5 Medium, With Vitamins
Fine powder, applied for 50/100L media preparation
Applications
For use in plant cell culture and micropropagation.
Components, mg per litre
|
Micro Elements |
|
|
CoCl2.6H2O |
0.025 |
|
CuSO4.5H2O |
0.025 |
|
FeNaEDTA |
36.70 |
|
H3BO3 |
3.0 |
|
KI |
0.75 |
|
MnSO4.H2O |
10.0 |
|
Na2MoO4.2H2O |
0.25 |
|
ZnSO4.7H2O |
2.0 |
|
Macro
Elements |
|
|
CaCl2 |
113.23 |
|
KNO3 |
2500.00 |
|
MgSO4 |
121.56 |
|
NaH2PO4 |
169.57 |
|
(NH4)2SO4 |
134.0 |
|
Vitamins* |
|
|
Myo-inositol |
100.0 |
|
Nicotinic acid |
1.0 |
|
Pyridoxine HCl |
1.0 |
|
Thiamine HCl |
10.0 |
The total
concentration of Micro and Macro elements including vitamins: 3150mg/l
|
Auxins (Optional)** |
|
|
2,4-D |
0.5 |
|
Sucrose
& Agar (Optional)** |
|
|
Sucrose |
20,000 |
|
Agar |
A.C.D. |
A.C.D.~ As Customers' Demands
Preparation
Suspend 3.09g of the Gamborg B5 medium WO Vitamins
(3.2g Gamborg B5 medium with Vitamins) into distilled water ^, Mix
well and make up to 1000ml. Heat with continuous stirring, Adjust
the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or
flasks. Sterilise in the autoclave at 121℃ for 15 minutes (the B5 media with Sucrose should be autoclaved at /115℃ for 20
minutes or might turn dark at over 115℃).
^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances vigorously.
Storage
Media powder is extremely hygroscopic and should be protected from
atmospheric moisture. If possible, the unused powder should be stored in a
sealed box with silica desiccant and refrigerated at 2-8°C.
* Please select the
product via the 'Media Type' drop-down box and purchase
** To request a quote and order a customised version,
please contact support@gmexpression.com
Due to Australian Customs restrictions for flammable & volatile chemicals, it is strongly recommended that international buyers email us to confirm shipping availability prior to purchase.
