Gamborg B5 medium | W / WO Vitamins | 50/100L

Gamborg B5 medium | W/WO Vitamins

 -Gamborg B5 Basal Salt Mixture, W/O Vitamins

 -Gamborg B5 Medium, With Vitamins

Fine powder, applied for 50/100L media preparation

Applications

For use in plant cell culture and micropropagation.

Components, mg per litre

The total concentration of Micro and Macro elements, including vitamins: 3203(3325)mg/l

  A.C.D.~ As Customers' Demands 

Preparation

Suspend 3.09g of the Gamborg B5 medium WO Vitamins (3.2g Gamborg B5 medium with Vitamins /3.325g if using NaH₂PO₄.7H₂O) into distilled water ^, mix well and make up to 1000ml. Heat with continuous stirring.  Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Sterilise in the autoclave at 121℃ for 15 minutes (the B5 media with Sucrose should be autoclaved at /115℃ for 20 minutes or might turn dark at over 115℃).

^ During preparation, avoid naked flames and do not mix the basal salt mixture with reducing substances vigorously.

Storage

Media powder is extremely hygroscopic and should be protected from atmospheric moisture. If possible, the unused powder should be stored in a sealed box with silica desiccant and refrigerated at 2-8°C.

*   Please select the product via the 'Media Type' drop-down box and purchase

** To request a quote and order a customised version, please contact support@gmexpression.com

Due to Australian Customs restrictions for flammable & volatile chemicals, it is strongly recommended that international buyers email us to confirm shipping availability prior to purchase.