Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium) | 250g (333L)

Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium; Modified BBM) |250g (can prepare 333L liquid medium)

Bold Basal Medium Modified with trace elements and replaced MoO3 with Sodium Molybdate.

Applications

Modified Bold Basal Medium is an axenic-culture maintenance medium in phycology/algae studies.

Commonly used for the cultivation of a variety of freshwater algae cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). Other algae species like Zygnemataceae(Zygogonium ericetorum) can also be grown in Bold’s Basal Medium(Stancheva et al., 2014) 

Components, mg per litre

Macro Elements

Sodium nitrate                                   250

Potassium phosphate monobasic   175

Potassium phosphate dibasic           75

Magnesium sulfate • 7H2O                   75

Calcium chloride dihydrate                   25

Sodium chloride                                   25

Potassium hydroxide                           31

EDTA, Disodium Salt                                63.61 

Micro Elements

Boric acid                                         11.42

Zinc sulfate • 7H2O                         8.82

Ferrous sulfate • 7H2O                         4.98

Cobalt nitrate • 6H2O                         0.49

Cupric sulfate • 5H2O                         1.57

Manganese chloride • 4H2O         1.44

Sodium Molybdate                                 1.19         

Trace Elements 

Nickel chloride • 6H2O            0.003

Potassium iodide                    0.003

Sodium selenite                    0.002

Stannic chloride                        0.001

Vanadium sulfate • 3H2O        0.0022

Preparation

Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 litre of ion-free water or MQ water along with 0.75 g of Modified Bold Basal Medium.

The final solution pH should be adjusted to 6.6 +/- 0.1 with 5M KOH.