Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium) | 250g (333L)
- Product Code: GMNB-BMFN01
- Availability: 4-6 weeks
Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium; Modified BBM) |250g (can prepare 333L liquid medium)
Bold Basal Medium Modified with trace elements and replaced MoO3 with Sodium Molybdate.
Applications
Modified Bold Basal Medium is an axenic-culture maintenance medium in phycology/algae studies.
Commonly used for the cultivation of a variety of freshwater algae cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). Other algae species like Zygnemataceae(Zygogonium ericetorum) can also be grown in Bold’s Basal Medium(Stancheva et al., 2014)
Components, mg per litre
Macro Elements
Sodium nitrate 250
Potassium phosphate monobasic 175
Potassium phosphate dibasic 75
Magnesium sulfate • 7H2O 75
Calcium chloride dihydrate 25
Sodium chloride 25
Potassium hydroxide 31
EDTA, Disodium Salt 63.61
Micro Elements
Boric acid 11.42
Zinc sulfate • 7H2O 8.82
Ferrous sulfate • 7H2O 4.98
Cobalt nitrate • 6H2O 0.49
Cupric sulfate • 5H2O 1.57
Manganese chloride • 4H2O 1.44
Sodium Molybdate 1.19
Trace Elements
Nickel chloride • 6H2O 0.003
Potassium iodide 0.003
Sodium selenite 0.002
Stannic chloride 0.001
Vanadium sulfate • 3H2O 0.0022
Preparation
Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 litre of ion-free water or MQ water along with 0.75 g of Modified Bold Basal Medium.
The final solution pH should be adjusted to 6.6 +/- 0.1 with 5M KOH.