Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium) | For 300L
- Product Code: GMNB-BMFN01
- Availability: 4-6 weeks
The 500X concentrated stock of Trace Elements is only available for domestic customers within Australia. Please note that this higher concentration is classified as Regulated Dangerous Goods and is available for Road Transport only.
Bold Modified Freshwater Nutrient Solution (Modified Bold Basal Medium; Modified BBM) | For preparing 300L of liquid medium
Bold Basal Medium Modified with trace elements and replaced MoO3 with Sodium Molybdate.
Applications
Modified Bold Basal Medium is an axenic-culture maintenance medium in phycology/algae studies.
Commonly used for the cultivation of a variety of freshwater algae cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). Other algae species like Zygnemataceae(Zygogonium ericetorum) can also be grown in Bold’s Basal Medium(Stancheva et al., 2014)
Components, mg per litre
|
Mixture A |
|
|
Macro Elements |
|
|
NaNO3 |
250 |
|
K2HPO4 |
75 |
|
KH2PO4 |
175 |
|
MgSO4 ·7H2O |
75 |
|
CaCl2 · 2H2O |
25 |
|
NaCl |
25 |
|
KOH |
31 |
|
Na2 · EDTA |
63.61 |
|
Micro Elements |
|
|
H3BO3 |
11.42 |
|
ZnSO4 ·7H2O |
8.82 |
|
FeSO4 ·7H2O |
4.98 |
|
Co(NO3)2 ·6H2O |
0.49 |
|
CuSO4 · 5H2O |
1.57 |
|
MnCl2 · 4H2O |
1.44 |
|
Na2MoO4 |
1.19 |
|
Stock B - Trace Elements |
(Order seperately) |
|
NiCl2 · 6H2O |
0.003 |
|
Potassium iodide |
0.003 |
|
Sodium selenite |
0.002 |
|
Stannic chloride |
0.001 |
|
Vanadium sulphate |
0.0022 |
Preparation
Dissolve 0.75g of Mixture A in 980ml of ion-free water or MQ water, add 20 mL of Stock B to make the media solution 1 litre. Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 litre of Bold Basal Media liquid.
The final solution pH should be adjusted to 6.6 +/- 0.1 with 5M KOH.
