Schenk and Hildebrandt Medium | W / WO Vitamins | 50/100L
- Product Code: GMNB-SH01
- Availability: In Stock
Schenk & Hildebrandt Medium W/WO Vitamins
- Schenk & Hildebrandt Basal Salt Mixture, W/O Vitamins
- Schenk & Hildebrandt Medium, With Vitamins
Fine powder, applied for 50/100L media preparation
Applications
For use in plant cell culture and micropropagation.
Components, mg per litre
|
Micro Elements |
|
|
CoCl2.6H2O |
0.1 |
|
CuSO4.5H2O |
0.2 |
|
FeNaEDTA |
19.8 |
|
H3BO3 |
5.0 |
|
KI |
1.0 |
|
MnSO4.H2O |
10.0 |
|
Na2MoO4.2H2O |
0.1 |
|
ZnSO4.7H2O |
1.0> |
|
Macro Elements |
|
|
CaCl2 |
151.0 |
|
KNO3 |
2500.0 |
|
MgSO4 |
195.05 |
|
(NH4)H2PO4 |
300.0 |
|
Vitamins* |
|
|
Myo-inositol |
1000.0 |
|
Nicotinic acid |
5.0 |
|
Pyridoxine HCl |
0.5 |
|
Thiamine HCl |
5.0 |
The total concentration of Micro and Macro elements including vitamins: 4193.75mg/l
|
Auxins (Optional)** |
|
|
2,4-D # |
0.5 |
|
4-CPA# |
2.0 |
|
Cytokin## |
0.1 |
|
Kinetin## |
0.1 |
# For monocotyledonous cell
## For dicotyledonous cell
|
Sucrose
& Agar (Optional)** |
|
|
Sucrose |
20000 |
|
Agar |
A.C.D. |
|
Element deletion |
A.C.D. |
A.C.D.~ As Customers' Demands
Preparation
Suspend 3.18g of the S&H medium WO Vitamins (or 4.19g S&H medium with Vitamins) into MQ or double-distilled water. Heat with continuous stirring till all dissolved. Make the volume to 1000ml. Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Autoclave at 121°C for 15 minutes (the S&H media with Sucrose should be autoclaved at 115°C for 20 minutes or might turn dark at over 115°C).
During preparation, avoid naked flames, and DO NOT vigorously mix the basal salt mixture with reducing substances such as sucrose in powder form, which may cause an explosion.
Note
- DO NOT intensively hit the medium powder, as it contains ammonium and nitrate which may cause an explosion.
- This mixture contains a large amount of strong oxidising ingredients. We pre-packed the powder in a PP bag, which you can remove after opening. If the powder directly contacts the bottle, the surface of the plastic bottle and cap may turn yellow/brown after long-term storage, which will not affect the medium.
- If possible, the unused powder should be stored in a sealed glass reagent bottle and refrigerated at 2~8°C.
- When the liquid media is prepared, please store it at 2~8°C, the shelf life of such a stock can be several years as all ingredients and elements are in a stable inorganic salt form.
* Please select the product via the 'Media Type' drop-down box and purchase
** To request a quote and order a customised version,
please contact support@gmexpression.com
Due to Australian Customs restrictions for flammable & volatile chemicals, it is strongly recommended that international buyers email us to confirm shipping availability prior to purchase.
