Schenk and Hildebrandt Medium | W / WO Vitamins | 50/100L
- Product Code: GMNB-SH01
- Availability: In Stock
Schenk & Hildebrandt Medium W/WO Vitamins
- Schenk & Hildebrandt Basal Salt Mixture, W/O Vitamins
- Schenk & Hildebrandt Medium, With Vitamins
Fine powder, applied for 50/100L media preparation
Applications
For use in plant cell culture and micropropagation.
Components, mg per litre
|
Micro Elements |
|
|
CoCl2.6H2O |
0.1 |
|
CuSO4.5H2O |
0.2 |
|
FeNaEDTA |
19.8 |
|
H3BO3 |
5.0 |
|
KI |
1.0 |
|
MnSO4.H2O |
10.0 |
|
Na2MoO4.2H2O |
0.1 |
|
ZnSO4.7H2O |
1.0> |
|
Macro Elements |
|
|
CaCl2 |
151.0 |
|
KNO3 |
2500.0 |
|
MgSO4 |
195.05 |
|
(NH4)H2PO4 |
300.0 |
|
Vitamins* |
|
|
Myo-inositol |
1000.0 |
|
Nicotinic acid |
5.0 |
|
Pyridoxine HCl |
0.5 |
|
Thiamine HCl |
5.0 |
The total concentration of Micro and Macro elements including vitamins: 4193.75mg/l
|
Auxins (Optional)** |
|
|
2,4-D # |
0.5 |
|
4-CPA# |
2.0 |
|
Cytokin## |
0.1 |
|
Kinetin## |
0.1 |
# For monocotyledonous cell
## For dicotyledonous cell
|
Sucrose
& Agar (Optional)** |
|
|
Sucrose |
20000 |
|
Agar |
A.C.D. |
|
Element deletion |
A.C.D. |
A.C.D.~ As Customers' Demands
Preparation
Suspend 3.18g of the S&H medium WO Vitamins (or 4.19g S&H medium with Vitamins) into MQ or double-distilled water. Heat with continuous stirring till all dissolved. Make the volume to 1000ml. Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Autoclave at 121°C for 15 minutes (the S&H media with Sucrose should be autoclaved at 115°C for 20 minutes or might turn dark at over 115°C).
During preparation, avoid naked flames, and DO NOT vigorously mix the basal salt mixture with reducing substances such as sucrose in powder form, which may cause an explosion.
Note
- DO NOT intensively hit the medium powder, as it contains ammonium and nitrate which may cause an explosion.
- This mixture contains a large amount of strong oxidizing ingredients. The surface of the plastic bottle and cap may turn yellow/brown after long-term storage, which will not affect the medium.
- If possible, the unused powder should be stored in a sealed glass reagent bottle and refrigerated at 2~8°C.
- We recommend the user use up all the mixture when first opening the bottle. Dissolve all the mixture in double distilled water or MQ water and make 1000 ml of 100X stock for future use. 0.22 μm filter sterilise is optional.
The 100X stock can be stored at 2~8°C, the shelf life of such a stock can be several years as all ingredients and elements are in a stable inorganic salt form.
* Please select the product via the 'Media Type' drop-down box and purchase
** To request a quote and order a customised version,
please contact support@gmexpression.com
Due to Australian Customs restrictions for flammable & volatile chemicals, it is strongly recommended that international buyers email us to confirm shipping availability prior to purchase.
