Knudson C Orchid Medium Basal Salt | WO Vitamins & Sucrose | 100L
- Product Code: GMNB-KNDC-S01
- Availability: 4-6 weeks
Tags: Knudson C Medium
This item is not usually in stock due to low daily demand and long-term storage challenges on raw materials.
Upon receiving your order, we will commence with the manufacturing process. The processing time will be approximately 4 to 6 weeks.
Knudson C Orchid Medium Basal Salt | WO Vitamins & Sucrose
Fine powder, applied for 100L media preparation
Applications
For use in plant cell culture and micropropagation.
Knudson C Orchid Medium consists of the macroelements and microelements as described by Knudson (1946) . The medium was originally developed for the in vitro germination of Cymbidium orchid seeds but can also be used for other orchid species.
It is a low-salt formulation of the inorganic salts and carbohydrate. Ammonium sulphate and calcium nitrate serve as sources of nitrogen and stimulate seed germination and differentiation. Microelements like Manganese, Molybdenum, Copper, Iron and Zinc enhance metabolism and help in germination. Boron plays a key role in carbohydrate metabolism.
Components, mg per litre
Micro Elements
Fe-EDTA trihydrate 42.11
Manganese sulfate monohydrate 5.68
Boric acid 0.06
Copper sulfate 0.04
Sodium molybdate dihydrate 0.034
Zinc sulfate monohydrate 0.218
Macro Elements
Ammonium nitrate 500.00
Calcium nitrate 694,8
Magnesium sulphate 122.09
Potassium phosphate monobasic 250.00
Vitamins（optional）*
Auxins（optional）**
Sucrose & Agar （optional）**
Sucrose (as carbohydrate) 20,000
Agar A.C.D.
Charcoal A.C.D. (suggest 2,000)
Banana Powder A.C.D. (suggest 25,000) (100,000 for fresh banana)
A.C.D.~ As Customers' Demands
Preparation
Suspend 1.62g of the Knudson C Orchid Medium Basal Salt into distilled water^, Mix well and make up to 1000ml. Heat with continuous stirring, Adjust the medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks. Sterilise in the autoclave at 121
