Chu N6 medium | W / WO Vitamins | 50/100L
- Product Code: GMNB-CN6M01
- Availability: In Stock
Tags: Chu N6 medium
Chu N6
medium | W/WO Vitamins
-Chu N6 Basal Salt Mixture, W/O Vitamins
-Chu N6 Medium, With Vitamins
Fine powder, applied for 50/100L media preparation
Applications
For use in plant cell culture, defined to improve the formation,
growth and differentiation of pollen callus in rice & cereal crops.
Components,
mg per litre
Micro Elements |
|
FeNaEDTA |
36.7 |
H3BO3 |
1.6 |
KI |
0.8 |
MnSO4.H2O |
3.33 |
ZnSO3.7H2O |
1.5 |
Macro Elements |
|
CaCl2 |
125.33 |
KH2PO3 |
400 |
KNO3 |
2830 |
MgSO4 |
90.27 |
(NH4)2SO4 |
463 |
Vitamins* |
|
Glycine |
2 |
Thiamine HCl |
1 |
Pyridoxine HCl |
0.5 |
Nicotinic acid |
0.5 |
Total concentration Micro and Macro elements
including -vitamins: 3956.53 mg/l
Sucrose & Agar (optional)** |
|
Sucrose |
20000 |
Agar |
A.C.D. |
Preparation
Suspend
3.95g of the Chu N6 medium W/O Vitamins (3.95~3.96g Chu N6 medium With
Vitamins) into distilled water^, Mix
well and make up to 1000ml. Heat with continuous stirring, Adjust the
medium to the desired pH using 1M HCl/NaOH and aliquot into tubes or flasks.
Sterilise in the autoclave at 121°C for 15 minutes (the media with Sucrose
should be autoclaved at 115°C for 20 minutes or might turn dark at over 115°C).
^
During preparation, avoid naked flames and do not mix the basal salt mixture
with reducing substances such as sucrose in powder form (they must be dissolved
in liquid in sequence when preparing).
Storage
Media
powder is extremely hygroscopic and should be protected from atmospheric
moisture. If possible, the unused powder should be stored in a sealed box with
silica desiccant and refrigerated at 2-8°C.
*
Please select the product via the 'Media Type' drop-down box and purchase
** To
request a quote and order a customised version, please contact
support@gmexpression.com
Due to
Australian Customs restrictions for flammable & volatile chemicals, it is
strongly recommended that international buyers email us to confirm shipping
availability prior to purchase.