Luria-Bertani Broth, LB media, Powder (Miller's) , 250g
- Product Code: GMNB-LBB115
- Availability: In Stock
LB Broth, culture media
250g, fine powder (Miller's), up to make 10L liquid media.
500g, fine powder (Miller's), up to make 20L liquid media.
LB is a microbial growth medium commonly used for the cultivation of E. coli. This broth contains peptides, amino acids, water-soluble vitamins and carbohydrates. It is suitable for the non-selective culture of E. coli strains for cloning, DNA plasmid preparation and expression of recombinant proteins. It is also suitable for selective cultures when appropriate antibiotics are added.
Components
Sodium chloride 10.0 g/L
Tryptone * 10.0 g/L
Yeast extract (premium) 5.0 g/L
* Vegetable tryptone can be arranged if requested in advance. The price is the same.
Final pH 7.0 ± 0.1 at 25 ℃
Preparation
Suspend 25.0 g of LB Broth (or with Agar for preparing solid media) in distilled water, mix well and make up to 1000 ml.
Sterilise by autoclaving at 121℃ for 15 minutes. Aliquot as needed.
