Gellan and pullulan composite hydrogel | 250g
- Product Code: GEL-GPHG01
- Availability: In Stock
Gellan and pullulan composite hydrogel
Pre-mixed powder | 250g
Separate package | 125g Gellan + 125g Pullulan
For the preparation of plant tissue high water retention culture media and quick-setting high elasticity bacterial culture media.
Components
Gellan（low acyl gellan） CAS: 71010-52-1
Pullulan CAS: 9057-02-7
Gellan and Pullulan Molar ratio 1:1（premix powder）
NaOH 100X 1 mM
CaCl2 100X 1 mM
Form
Light yellow fine powder.
Properties
| Loss on drying | ≤20% |
| Residual on ignition | ≤5% |
| Starch test | Pass |
| The density test of microbial colonies | Pass |
| Water absorption | ~95ml/(g dry gel) |
| Gel strength (10g/L, 20°C) | ≥1900g/cm2 |
| Curing temperature | 35-45°C |
| Shelf life | 3 years |
Caution
Strong acids and prolonged heating may cause the polysaccharide molecules to break down into oligosaccharides and lose their physical properties.
Preparation
This product is available in two packaging forms, per-mixed powder for the preparation of equal proportions of gels (follow 3a as below), and separated packaged for the preparation of customized gels (follow 3b as below)
1. Prepare nutrition media stock solution, like MS or LB, 2~5X Stock suggested.
2. Prepare CaCl2 and NaOH 100X stock separately, sterilise with 0.22um filter.
3a. Dissolve 20~40g (1~2% gel) pullulan & gellan per-mix powder in about 400~700ml preheated MQ water ( ~80°C) while stirring the solution at 600 rpm to obtain a homogeneous solution, sterilise with 0.22um filter*.
3b. Pullulan & gellan stock solution (4%) was prepared separately by dissolving in lukewarm water (>50°C) and stirring at 600 rpm to ensure a complete solution. Sterilise through a 0.22 um filter*.
4. Swiftly mix the polysaccharide solution (pre-mix solution or separate stock) with CaCl2 and NaOH 100X stock, make up to 1000 ml by adding MQ water or nutrition media Stock to a final concentration of 0.01 mM CaCl2 and 0.01 mM NaOH, resting for 1 to 2 minutes at room temperature until the gel fully cured.
*When using the autoclaving method, we recommend sterilization at 113 degrees for 25 minutes.
