Double yeast extract medium | 2x YT | 250g
AUD 57.00
Excl.GST
- Product Code: GMNB-2YE01
- Availability: In Stock
Tags: 2x YT media
2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Components
16 g/L Tryptone
10 g/L Yeast extract (premium)
5.0 g/L NaCl
10 g/L Yeast extract (premium)
5.0 g/L NaCl
Preparation
Suspend 31.0 g of 2x YT (or with Agar for preparing solid media) in distilled water, mix well and make up to 1000 ml.
Sterilize by autoclaving at 121℃ for 15 minutes. Aliquot as needed.
