Double yeast extract medium, 2x YT | 250g
AUD 57.00
Excl.GST
- Product Code: GMNB-2YE01
- Availability: In Stock
Tags: 2x YT media
Double yeast extract medium, 250 g
2xYT is a standard microbial growth medium used for the cultivation of
E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Suitable for non-selective cultivation of M13 bacteriophage, and
E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
The yeast extract we use is derived from high-quality baking yeast that is completely free from tannic acid contamination — an element that can inhibit bacterial growth, which results in a product that outperforms conventional wine/beer yeast extracts. Additionally, compared to the major competing supplier, we use a superior beef‑sourced tryptone. This ingredient delivers a well-balanced amino acid profile along with essential fatty acids and organic iron, promoting the growth of fastidious bacteria more effectively than vegetable peptone (typically soy peptone), which is often chosen for its lower cost and easier international customs clearance despite its higher lectin content, which also interfere the sensertive bacteria growth.
Components
16 g/L Tryptone
10 g/L Yeast extract (premium)
5.0 g/L NaCl
10 g/L Yeast extract (premium)
5.0 g/L NaCl
Preparation
Suspend 31.0 g of 2x YT (or with Agar to prepare solid media) in distilled water, mix well, and make up to 1000 ml.
Sterilise by autoclaving at 121℃ for 15 minutes. Aliquot as needed.
