DNA Booster 50X Stock| without LB | 50ml

DNA Booster 50X Stock（v1.2*）| without LB | 50ml

Preparation of up to 2.5 litres of liquid medium

0.22μm filtration plus 5min 275/365nm UV treatment sterilized.

This is a unique Media Enhancement Stock developed by GAMES (General Molecular Expression Service Pty Ltd). It has been designed to increase the yield of the bacterial plasmid for the most commonly used liquid media.

It gives an optimum yield of transformed bacteria in culture, resulting in a much higher plasmid yield per volume compared to traditional LB media. 

Recommended to use

• Suitable for the preparation of plasmids of bacteria such as E. coli in the laboratory.
• It is suitable for the recovery of electroporation-transformed bacteria.
• Also suitable for the preparation of competent cells.
  

Composition

Booster formula

High-density cell growth maintenance component (GMES’s confidential)

Plasmid/gDNA Anabolic Enhancement Component (GMES‘s confidential)

Preparation

Preparation

·    For high-copy number plasmid preparation, use the dark colour stock, and for low-copy number plasmid uses the light colour one.

• After opening, the remaining liquid should be sealed immediately and stored in the 4°C(39.2 ℉) refrigerator. Operation on a sterile bench is recommended.
• Users need to prepare either LB, SOB or 2YT sterilized liquid media in advance.
  
• Dilute 1 volume of 50X stock to 49 volumes of liquid media (LB, SOB or 2YT;  e.g. add 10 ml of 50X stock to 490 ml of LB medium to make 500 ml DNA Booster medium), shake well and use in the same way as a normal LB medium. For E.coli (like TOP10, DH5a strain) incubation,  Incubate for 2-3 hours to obtain highly active bacteria (in the log phase of growth), for 4 hours of rapid incubation (plateau growth) or for overnight incubation.

Caution

This stock solution should not be autoclaved. It should only be sterilised by 0.22µm filtration.

*The formulas for our proprietary products are constantly updated as our back-end research continues, with newer versions of the products being released to the shelves when the results of subsequent development show improved performance.

**The data shown in the figure is the actual performance of version 1.0 product (as of February 2023), version 1.2 product has a 10~20% better performance compared to version 1.0.