DNA Booster | plasmid specific medium | 500ml

DNA Booster  v1.2*| plasmid specific medium | 500ml

0.22μm filtration plus 5min 275/365nm UV treatment sterilized.

This is a unique medium developed by GMES (General Molecular Expression Service Pty Ltd) using LB as the base formulation.

It gives an optimum yield of transformed bacteria in culture, resulting in a much higher plasmid yield per volume compared to LB media. 

Recommended to use

• Suitable for the preparation of plasmids of bacteria such as E. coli in the laboratory.
• It is suitable for the recovery of electroporation transformed bacteria.
• Also suitable for the preparation of competent cells.
  

Composition

LB base formula

Sodium chloride 10.0 g/L

Tryptone (vegetal) 10,0 g/L

Yeast extract (premium) 5.0 g/L

Booster formula

High-density cell growth maintenance component (GMES’s confidential)

Plasmid/gDNA Anabolic Enhancement Component (GMES‘s confidential)

Preparation

For high-copy number plasmid preparation, please purchase the high-copy number version, and for low-copy number plasmid use the low-copy number one.

- Use directly after opening, the remaining liquid should be sealed immediately and stored in the 4°C(39.2 ℉) refrigerator. Operation on a sterile bench is recommended.

- Use this medium in the same way as a normal LB medium. For E.coli (like TOP10, DH5a strain) incubation,  Incubate for 2-3 hours to obtain highly active bacteria (in the log phase of growth), for 4 hours of rapid incubation (plateau growth) or for overnight incubation.

Caution

This mixed medium should not be autoclaved. It has already been sterilised and should only be re-sterilised by 0.22µm filtration (in case of contamination).

*The formulas for our proprietary products are constantly updated as our back-end research continues, with newer versions of the products being released to the shelves when the results of subsequent development show improved performance.

**The data shown in the figure is the actual performance of version 1.0 product (as of February 2023), version 1.2 product has a 10~20% better performance compared to version 1.0.

Current promotional offer.

A 10ml free trial sample of this medium would be given to customers who purchase our medium or buffer products.