DNA Booster | plasmid specific medium | 10ml free trial
- Product Code: DBV-01EP
- Availability: In Stock
Tags: DNA Booster
This is a free trial for customers who purchase culture media and/or buffer solutions.
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DNA Booster v1.2*| plasmid specific medium | 10ml experience packaging
0.22μm filtration plus 5min 275/365nm UV treatment sterilized.
This is a unique medium developed by GMES (General Molecular Expression Service Pty Ltd) using LB as the base formulation.
It gives an optimum yield of transformed bacteria in culture, resulting in a much higher plasmid yield per volume compared to LB media.
Recommended to use
- Suitable for the preparation of plasmids of bacteria such as E. coli in the laboratory.
- It is suitable for the recovery of electroporation transformed bacteria.
- Also suitable for the preparation of competent cells.
Composition
LB base formula
Sodium chloride 10.0 g/L
Tryptone (vegetal) 10,0 g/L
Yeast extract (premium) 5.0 g/L
Booster formula
High-density cell growth maintenance component (GMES’s confidential)
Plasmid/gDNA Anabolic Enhancement Component (GMES‘s confidential)
Preparation
- Use directly after opening, the remaining liquid should be sealed immediately and stored in the 4°C(39.2 ℉) refrigerator. Operation on a sterile bench is recommended.
- Use this medium in the same way as a normal LB medium. For E.coli (like TOP10, DH5a strain) incubation, Incubate for 2-3 hours to obtain highly active bacteria (in the log phase of growth), for 4 hours of rapid incubation (plateau growth) or for overnight incubation.
*The formulas for our proprietary products are constantly updated as our back-end research continues, with newer versions of the products being released to the shelves when the results of subsequent development show improved performance.
**The data shown in the figure is the actual performance of version 1.0 (as of February 2023) of the product.