Tryptic Soy Broth, TSB , 500g

Tryptic Soy Broth, TSB

500g, fine powder, up to make 16L liquid media.

Aliases: Soybean Casein Digest Medium, Tryptone Soya Broth

General-purpose liquid culture medium, supports the growth of a broad range of aerobic and facultative anaerobic bacteria and fungi. It is composed of enzymatic digests of soybean meal and casein, which provide essential amino acids and nitrogenous compounds. TSB is frequently used for sterility testing, cultivating fastidious microorganisms, and as a pre-enrichment broth for various microbial tests in the food, pharmaceutical, and clinical industries.

Common Applications

Sterility Testing

Detects microbial contamination in pharmaceuticals, cosmetics, and medical devices.

Cultivation of Microorganisms

Supports growth of fastidious and non-fastidious organisms, including biofilm-forming strains.

Pre-enrichment

Used in food and clinical microbiology to enhance recovery of stressed or low-level organisms.

Inoculum Preparation

Ideal for preparing standardized inocula for antimicrobial susceptibility testing.

Biofilm Studies

Facilitates biofilm formation for structural and resistance analysis.

Components

Sodium chloride                                                     5.0 g/L

Soya peptone                                                         3.0 g/L

Casein peptone                                                      17.0 g/L

Dipotassium hydrogen phosphate                        2.5 g/L

Glucose                                                                   2.5 g/L

Final pH 7.3 ± 0.2 at 25°C

For Petri dishes: add 1.5% w/v Agar.         

Preparation

Dissolve 30.0 g of media powder in 1L of distilled water or MQ water. Mix thoroughly.

Adjust pH to 7.1~7.5.

Autoclave at 121°C for 15 minutes. 

Store prepared media at 2~6°C, protected from direct light.