Modified Chopped Meat Broth Medium (ATCC Medium 1490, ATCC Medium 2124) | For 5L
- Product Code: GMNB-ATCC-CMB01
- Availability: Pre-Order
Modified Chopped Meat Broth Medium (ATCC Medium 1490), for 5L
For bulk buy, please contact us for a quote via email support@gmexpression.com.
Applications
Modified Chopped Meat Broth Medium (ATCC Medium 1490) is suitable for:
- Pre-reduced, anaerobically sterilised (PRAS) media applications
- Cultivation, maintenance, and metabolic profiling of spore-forming and non-spore-forming obligate anaerobes
- Use as a holding medium for anaerobic cultures
- Promoting sporulation and toxin production in Clostridium species
- Gas-liquid chromatography (GLC) analysis of bacterial metabolic end products
|
Package contains |
5L pack |
|
Defatted Ground Beef |
500.0 g |
|
Mixture A |
200.0 g |
|
Mixture B |
12.8 g |
|
Stock C (0.22µm filter sterilised) |
100 ml |
Components
|
Defatted Ground Beef (Fat-Free), Freeze-Dried Granules* |
*Certified South Australian grass‑fed, antibiotic‑free cattle source, with optional autoclave treatment for strict import compliance. |
|
Mixture A |
per 1000 ml |
|
Casitone (Casein peptone) |
30.0 g |
|
Yeast Extract (Premium Grade) |
5.0 g |
|
Dipotassium Phosphate (K₂HPO₄) |
5.0 g |
|
Resazurin |
1.0 mg |
|
Mixture B |
per 1000 ml |
|
Sodium Bicarbonate (NaHCO₃) |
2.0 g |
L-Cysteine·HCl |
0.5 g |
Hemin |
5.0 mg |
|
Stock C |
per 1000 ml |
|
Vitamin K₁ in Ethanol 0.05 mg/mL [1] |
20 ml |
|
Optional stock & component |
per 1000 ml |
Clindamycin Hydrochloride Stock 1 mg/mL [2] |
5 ml |
Pre-Reduced Mixture [3] |
20 g |
Defatted Beef Heart Extract [4] |
20 g |
[1] Optional alternatives:
- Vitamin K₃ Stock
- Viscous liquid state of 98% Vitamin K₁ that has a longer shelf life at RT is also available, reagent pre-aliquoted into centrifuge tubes, each containing a sufficient amount to prepare a 20 mL stock solution at a final concentration of 0.05 mg/mL.
[2] 0.22µm filter sterilised. Adding Clindamycin Hydrochloride Stock will achieve ATCC medium 2124, Modified chopped meat medium with clindamycin. Select the corresponding option to purchase.
[3] Adding 10g/L of Iron Filings, Dextrose 5.0g/L, and Sodium Chloride 5.0g/L will achieve Pre-reduced Chopped Meat medium, used specifically for culturing Clostridium species like Clostridium perfringens (ATCC® 13124) and Bacteroides fragilis (ATCC® 25285); Streptococcus pyogenes (ATCC® 19615). Select the corresponding option to purchase.
[4] Replacing Defatted Ground Beef with Defatted Beef Extract will achieve ATCC medium 2210, Enriched Anaerobe medium.
Preparation
- Dissolve 100 g defatted ground beef granules and 40 g of Mixture A powder in 980 mL of Milli-Q water and mix well
- Boil the broth in a water bath or microwave for 15 minutes, then cool the mixture under an anaerobic gas atmosphere (e.g., N₂ -80% / H₂ -10% / CO₂ -10%, or N₂ -100%).
- Add 2.5 g of Mixture B powder and 20 mL of Stock C
- Adjust the pH to 7.0.
- Dispense 7 mL into Hungate tubes or 1/5–1/4 volume of anaerobic culture bottles containing meat particles (1 part meat to 4–5 parts fluid) under the same anaerobic gas phase.
- Autoclave at 121 °C for 30 minutes.
For a solid medium, add 15.0g Agar. For a semi-solid medium, add 0.5g Agar. Dissolve together with Mixture A, to make 1000 mL of medium.
Storage
- Unopened Granules: Store at –20 °C in a dry, light-protected environment. For extended shelf life, –65 °C is recommended. Shelf life: 2~3 years.
- Opened Granules: Immerse in 25 mM NaOH solution and store at –20 °C. Shelf life: 3 months.
- Stock C: Store in a brown bottle at 2~8 °C, protected from light. Shelf life: 6 months.
