Peptone Yeast Extract Glucose Broth (PYG) | For 6L

Peptone Yeast Glucose (PYG) Broth / Remel PYG Broth, for 6L

Product Description

Peptone Yeast Glucose (PYG) Broth is a pre‐reduced, anaerobically sterilised (PRAS) medium designed for the maintenance, enumeration, and physiological studies of anaerobic bacteria. Enriched and nonselective, it is supplemented with hemin and vitamin K to support fastidious anaerobes such as Prevotella spp., Porphyromonas spp., and the Bacteroides fragilis group. By incorporating specific biochemicals, PYG Broth can be used to assess utilisation, stimulation, or inhibition of target metabolic pathways.

Per 1000mL of PYG Broth contains

Final pH: 7.0 ± 0.2

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Preparation

1. Dissolve  40.1 g of Bottle A powder in 980 mL of Milli-Q water and mix well
2. Boil the broth in a water bath or microwave for 15 minutes, and then cool the mixture under an anaerobic gas atmosphere (e.g., 80% N₂, 10% H₂, 10% CO₂, or 100% N₂).
3. Add 0.95 g of Bottle B powder and 20 mL of Stock C
4. Adjust the pH to 7.0.
5. Dispense 7 mL into Hungate tubes or 1/5–1/4 volume of anaerobic culture bottles containing meat particles (1 part meat to 4–5 parts fluid) under the same anaerobic gas phase.
6. Autoclave at 121 °C for 30 minutes.
7. For a solid medium, add 15.0g Agar. For a semi-solid medium, add  0.5g Agar, dissolve together with powder A, to make 1000 mL of medium.

Storage and Handling

Store the dry powder in a cool, dry place. Once reconstituted, maintain anaerobic conditions throughout use. Ensure solutions of vitamin K are filter-sterilised and protected from light.