Gifu Anaerobic Medium, GAM | 250g
- Product Code: GMNB-GAM01
- Availability: In Stock
Tags: GAM Medium
GAM Medium (Gifu Anaerobic Medium) , 250g
GAM medium, For the culture of anaerobic bacteria.
Please refer to the Anaerobic Preparation Kit if you need any extra tools for anaerobic media preparation.
Per 1000mL of GAM Medium contains
| Proteose peptone | 10.0 g |
| Soy peptone | 3.0 g |
| Yeast extract | 5.0 g |
| Beef extract | 2.2 g |
| Digestive serum powder | 13.5 g |
| Beef liver powder | 1.2 g |
| Glucose | 3.0 g |
| Potassium dihydrogen phosphate | 2.5 g |
| Sodium chloride | 3.0 g |
| Soluble starch | 5.0 g |
| L-cysteine | 0.3 g |
| Sodium thioglycolate | 0.3 g |
PH 7.3±0.1 at 25℃
Preparation
Weigh 49.0g of this product, heat and dissolve in 1000ml of distilled water, autoclave at 121℃ for 15 minutes, cool to about 50℃, add 1ml of sterile 0.1% vitamin K1 solution and 1ml of Hemin solution (5mg/ml) to every 1000ml of the medium, mix well.
Note:
Add an appropriate amount of agar to make a semi-solid or solid culture medium.
Heating to dissolve the medium powder in distilled water is necessary. If skip heating, the prepared medium may form white colloidal precipitates after autoclave when cooled down.
Please select the optional stocks if vitamin K1 solution 30ml and Hemin solution 30ml are needed. Stocks are order-based and require 2 ~ 5 business days for preparation.
