Yeast Synthetic Drop-out Medium, without His-Met | SD-His-Met Medium, SD-HM | 250g

Yeast Synthetic Drop-out Medium, without His-Met (Yeast double Defective Medium, SD-Dropout Medium, SD-His-Met Medium, SD-HM)

Notice

This media is prepared in small batches and will be prepared after the purchase, taking around 5 to 10 business days for handling.

Product Description:

Yeast SD-His-Met Defective Medium, also known as SD-HM Medium, is a synthetic drop-out medium for screening nutritionally-deficient Saccharomyces cerevisiae for use in yeast protein expression, yeast hybrid, double-hybrid system, and so on.

SD deficient medium lacks corresponding components compared to yeast complete medium, which results in yeast strains that cannot synthesize the corresponding components and not being able to grow in the related defective type of medium.

This product does not contain sugar, and users can add glucose, raffinose or galactose according to the purpose of the experiment.

Use

SD-HM is used to screen for nutritionally deficient Saccharomyces cerevisiae.

SD-HH can be used as a protein expression medium for Saccharomyces cerevisiae when galactose is added.

SD-HM added with glucose can be used as a medium for the enrichment of defective yeast.

Since the composition of media is known, it can be used to identify nutritionally deficient types of Saccharomyces cerevisiae.

Storage:  kept in a cool and dry place.

Product properties: light yellow powder. when in the form of liquid: light amber, transparent, no precipitation or slight precipitation (insoluble calcium sulphate).

Components, per litre

Synthetic Dropout minimal base (SD):

Yeast base nitrogen source (YNB) 1.7g

Ammonium sulfate 5g

Drop-Out amino acids supplement (DO):

L-Arginine 50 mg

L-Aspartic Acid 80 mg

L-Isoleucine 50 mg

L-Leucine 100 mg

L-Lysine 50 mg

L-Phenylalanine 50 mg

L-Threonine 200 mg

L-Tryptophan 50 mg

L-Tyrosine 50 mg

L-Valine 150 mg

Adenine 40 mg

Uracil 20 mg

Preparation

[Fast preparation] 

Weigh 0.89 g ^[1] of mixture B (DO), suspend with 6.7 g of mixture A (SD), plus a suitable amount of agar ^[2] and sugar ^[3] in distilled or Milli-Q Water, make up to 1000ml.

PH adjustment is not usually required, but if the MQ water used is particularly acidic, adjust the pH to 5.8 before autoclaving.

Sterilise by autoclaving at 121°C for 15 minutes ^[4]. Aliquot as needed.

[1] Deduct the weight of defective amino acid from the total weight - 0.93 g. 

[2] 10~15g Agar for making agar plates.

[3] optionally add a suitable amount and appropriate type of carbon source.

[4] If equipment allows, autoclave at 115°C for 30 minutes to prevent the darkening of the solution due to a Maillard reaction on sugar catalysed by ammonium sulphate and heat. 

[Standard preparation]  

1. Prepare 0.5 litre of 10x SD stock solution:

Dissolve 33.5 g mixture A (SD) and optionally a suitable amount of sugar ^[1][2] in distilled or Milli-Q water and mix well, make up to 500 ml ^[3]. Sterilise by 0.22µm filtration (recommended) or autoclave at 115°C for 30 minutes.  Store the stock at room temperature. The shelf life of this solution is approximately one year.

2. Weigh 0.89 g of mixture B (DO), suspend with a suitable amount of agar in distilled or Milli-Q Water, make up to 900ml ^[3]. Sterilise by autoclaving at 121°C for 15 minutes.

3. Add 100 mL of sterile 10x SD stock into the freshly autoclaved (no longer boiling) mixture B (DO) solution, mix well and pour the plates while hot.  Store the SD-HM medium agar plates at 4°C. The shelf life is several months.

[1] Optionally add a suitable amount and appropriate type of carbon source. For example, add 100g dextrose for a final concentration of 20 g/L.

[2] Or add pre-prepared sugar stock, e.g. 20% w/v dextrose stock (Sterilised by 0.22µm filtration) also works.

[3] PH adjustment is not usually required, but if the MQ water used is particularly acidic, adjust the pH to 5.8 before autoclaving or filtering sterilise.

----------------

We provide a variety of customised yeast SD-Dropout media. The catalogues are listed below, and customers can request a quote and order less commonly used SD-Dropout media via support@gmexpression.com

Yeast SD Defective Medium （corresponding defective component is removed from the complete medium formulation):

SD complete medium

SD Single Defective Medium:

SD-Ura medium

SD-His medium

SD-Leu medium

SD-Met medium

SD-Ade medium

SD-Trp medium

SD Double Defective Medium:

SD-Ura-His medium

SD-Ura-Leu Medium

SD-Ura-Met medium

SD-Ura-Ade medium

SD-Ura-Trp medium

SD-His-Leu medium

SD-His-Met medium

SD-His-Ade medium

SD-His-Trp medium

SD-Leu-Met medium

SD-Leu-Ade medium

SD-Leu-Trp medium

SD-Met-Ade medium

SD-Met-Trp Medium

SD-Ade-Trp Medium 

SD Triple Deletion Medium:

SD-Ura-His-Leu Medium

SD-Ura-His-Met medium

SD-Ura-His-Ade medium

SD-Ura-His-Trp Medium

SD-Ura-Leu-Met medium

SD-Ura-Leu-Ade medium

SD-Ura-Leu-Trp Medium

SD-Ura-Met-Ade medium

SD-Ura-Met-Trp Medium

SD-Ura-Ade-Trp Medium

SD-His-Leu-Met Medium

SD-His-Leu-Ade medium

SD-His-Leu-Trp Medium

SD-His-Met-Ade medium

SD-His-Met-Trp Medium

SD-His-Ade-Trp medium

SD-Leu-Met-Ade Medium

SD-Leu-Met-Trp Medium

SD-Met-Ade-Trp Medium

SD four-deficient medium:

SD-Ura-His-Leu-Met medium

SD-Ura-His-Leu-Ade medium

SD-Ura-His-Leu-Trp Medium

SD-Ura-His-Met-Ade medium

SD-Ura-His-Met-Trp Medium

SD-Ura-His-Ade-Trp medium

SD-Ura-Leu-Met-Ade Medium

SD-Ura-Leu-Met-Trp medium

SD-Ura-Leu-Ade-Trp medium

SD-Ura-Met-Ade-Trp medium

SD-His-Leu-Met-Ade medium

SD-His-Leu-Met-Trp medium

SD-His-Leu-Ade-Trp medium

SD-His-Met-Ade-Trp medium

SD-Leu-Met-Ade-Trp Medium

SD Five-Deficient Medium:

SD-Ura-His-Leu-Met-Ade Medium

SD-Ura-His-Leu-Met-Trp medium

SD-Ura-His-Leu-Ade-Trp medium

SD-Ura-His-Met-Ade-Trp medium

SD-Ura-Leu-Met-Ade-Trp medium

SD-His-Leu-Met-Ade-Trp medium

SD Six Defects Medium:

SD-Ura-His-Leu-Met-Ade-Trp Medium