YPDS Liquid Medium ( Yeast Extract Peptone Dextrose Sorbitol) | 500mL

AUD 95.00    Excl.GST
  • Product Code: GMNB-YPDS111L
  • Availability: In Stock

Tags: Liquid YPDS

YPDS Liquid Medium (Yeast Extract Peptone Dextrose - Sorbitol) | 500mL

0.22μm filtration plus 5min 275/365nm UV treatment sterilized, Suggest storage in 4°C / 39.2 ℉  refrigerator.


For the preparation of Saccharomyces cerevisiae/Pichia pastoris competent cell

For Saccharomyces cerevisiae/Pichia pastoris Agar plate selection


Components 

YPDS medium, per litre 


Yeast extract (premium)   10g 

Peptone                            20g 

Glucose                            20g 

Sorbitol                             182.2g

pH  6.5±0.2 



Preparation

For the preparation of the selection agar plate

Add 10g agar and a suitable amount of antibiotics (such as Zeocin) to 500 ml YPDS Liquid, heat to 60~65°C and Stir well.  Aliquot into plates as needed. Operation on a sterile bench is recommended.


For the preparation of competent cell

Use directly after opening, the remaining liquid should be sealed immediately and stored in the 4°C refrigerator. Operation on a sterile bench is recommended.