YPDS Liquid Medium ( Yeast Extract Peptone Dextrose Sorbitol) | 500mL
- Product Code: GMNB-YPDS111L
- Availability: In Stock
Tags: Liquid YPDS
YPDS Liquid Medium (Yeast Extract Peptone Dextrose - Sorbitol) | 500mL
0.22μm filtration plus 5min 275/365nm UV treatment sterilized, Suggest storage in 4°C / 39.2 ℉ refrigerator.
For the preparation of Saccharomyces cerevisiae/Pichia pastoris competent cell
For Saccharomyces cerevisiae/Pichia pastoris Agar plate selection
Components
YPDS medium, per litre
Yeast extract (premium) 10g
Peptone 20g
Glucose 20g
Sorbitol 182.2g
pH 6.5±0.2
Preparation
For the preparation of the selection agar plate
Add 10g agar and a suitable amount of antibiotics (such as Zeocin) to 500 ml YPDS Liquid, heat to 60~65°C and Stir well. Aliquot into plates as needed. Operation on a sterile bench is recommended.
For the preparation of competent cell
Use directly after opening, the remaining liquid should be sealed immediately and stored in the 4°C refrigerator. Operation on a sterile bench is recommended.
