• Yeast Synthetic Drop-out Medium, SD single-defective Medium

Yeast Synthetic Drop-out Medium, SD single-defective Medium

AUD 199.00    Excl.GST
  • Product Code: GMNB-SDWO01
  • Availability: In Stock

Tags: SD Single Defective Medium

Yeast Synthetic Drop-out Medium, SD-Dropout Medium: single-defective medium


Packed for making 32 L of media solution:

Bottle A - Synthetic Dropout minimal base (SD): 220 g

Bottle B - Drop-Out amino acids supplement (DO): 30 g minus the weight of defective amino acid


Notice

This media is prepared in small batches and will be prepared after the purchase, taking 5 to 10 business days for handling.


Notice

This media is prepared in small batches and will be prepared after the purchase, taking 5 to 10 business days for handling.


Product Description:

Synthetic drop-out medium is used to screen various nutritionally deficient types of Saccharomyces cerevisiae for yeast protein expression, yeast hybrid, double-hybrid system, etc.

SD deficient medium lacks corresponding components compared to yeast complete medium, which results in yeast strains that cannot synthesize the corresponding components and not being able to grow in the related defective type of medium.

This product does not contain sugar. Users can add glucose, raffinose, or galactose for different experiment purposes.


Use

SD single-defective Medium is used to screen for nutritionally deficient Saccharomyces cerevisiae.

SD single-defective Medium can be used as a protein expression medium for Saccharomyces cerevisiae when galactose is added.

SD single-defective Medium added with glucose can be used as a medium for the enrichment of defective yeast.

Upon the composition of SD single-defective Medium, it can be used to identify nutritionally deficient types of Saccharomyces cerevisiae.


Storage: in a cool and dry place.

Product properties: light yellow powder. when in the form of liquid: light amber, transparent, no precipitation or slight precipitation (insoluble calcium sulphate).


Components, per litre


Synthetic Dropout minimal base (SD):

Yeast base nitrogen source (YNB) 1.7g

Ammonium sulphate 5g


Drop-Out amino acids supplement (DO):

L-Arginine 50 mg

L-Aspartic Acid 80 mg

L-Histidine 20 mg -- to be removed upon medium type

L-Isoleucine 50 mg

L-Leucine 100 mg -- to be removed upon medium type

L-Lysine 50 mg

L-Methionine 20 mg -- to be removed upon medium type

L-Phenylalanine 50 mg

L-Threonine 200 mg

L-Tryptophan 50 mg -- to be removed upon medium type

L-Tyrosine 50 mg

L-Valine 150 mg

Adenine 40 mg -- to be removed upon medium type

Uracil 20 mg -- to be removed upon medium type


Preparation

[Fast preparation] 

Dissolve a correct amount [1] of mixture B (DO), 6.7 g of mixture A (SD), and a suitable amount of agar [2] and sugar [3] in distilled or Milli-Q Water, make it to 1000ml.

PH adjustment is not usually required, but if the MQ water used is particularly acidic, adjust the pH to 5.8 before autoclaving.

Sterilise by autoclaving at 121°C for 15 minutes [4]. Aliquot as needed.


[1] Deduct the weight of defective amino acids from the total weight of 0.93 g. For example, for SD-His, it will be 0.93 g - 0.02 g = 0.91 g.

[2] 10~15g Agar for making agar plates.

[3] optionally add a suitable amount and appropriate type of carbon source.

[4] If equipment allows, autoclave at 115°C for 30 minutes to prevent the darkening of the solution due to a Maillard reaction on sugar catalysed by ammonium sulphate and heat. 


[Standard preparation]  

1. Prepare 0.5 litre of 10X SD stock solution:

Dissolve 33.5 g mixture A (SD) and optionally a suitable amount of sugar [1][2] in distilled or Milli-Q water and mix well, make up to 500 ml [3]. Sterilise by 0.22µm filtration (recommended) or autoclave at 115°C for 30 minutes.  Store the stock at room temperature. The shelf life of this solution is approximately one year.


2. Dissolve a correct amount [4] of mixture B (DO), and a suitable amount of agar, in distilled or Milli-Q Water, make it to 900ml [3]. Sterilise by autoclaving at 121°C for 15 minutes.


3. Add 100 ml of sterile 10x SD stock into the freshly autoclaved (no longer boiling) mixture B (DO) solution, mix well, and pour the plates while hot.  Store the SD single-defective medium agar plates at 4°C. The shelf life is several months.


[1] Optionally add a suitable amount and appropriate type of carbon source. For example, add 100g dextrose for a final concentration of 20 g/L.

[2] Or add pre-prepared sugar stock, e.g. 20% w/v dextrose stock (Sterilised by 0.22µm filtration) also works.

[3] PH adjustment is not usually required, but if the MQ water used is particularly acidic, adjust the pH to 5.8 before autoclaving or filtering sterilise.

[4] Deduct the weight of defective amino acid from the total weight of 0.93 g. For example, for SD-His, it will be 0.93 g - 0.02 g = 0.91 g.


----------------

We provide a variety of customised yeast SD-Dropout media. The catalogues are listed below, and customers can request a quote and order less commonly used SD-Dropout media via support@gmexpression.com


Yeast SD Defective Medium (corresponding defective component is removed from the complete medium formulation):


SD complete medium (view product)


SD Single Defective Medium:

SD-Ura medium

SD-His medium

SD-Leu medium

SD-Met medium

SD-Ade medium

SD-Trp medium


SD Double Defective Medium: (view product)

SD-Ura-His medium

SD-Ura-Leu Medium

SD-Ura-Met medium

SD-Ura-Ade medium

SD-Ura-Trp medium

SD-His-Leu medium

SD-His-Met medium

SD-His-Ade medium

SD-His-Trp medium

SD-Leu-Met medium

SD-Leu-Ade medium

SD-Leu-Trp medium

SD-Met-Ade medium

SD-Met-Trp Medium

SD-Ade-Trp Medium 


SD Triple Deletion Medium:

SD-Ura-His-Leu Medium

SD-Ura-His-Met medium

SD-Ura-His-Ade medium

SD-Ura-His-Trp Medium

SD-Ura-Leu-Met medium

SD-Ura-Leu-Ade medium

SD-Ura-Leu-Trp Medium

SD-Ura-Met-Ade medium

SD-Ura-Met-Trp Medium

SD-Ura-Ade-Trp Medium

SD-His-Leu-Met Medium

SD-His-Leu-Ade medium

SD-His-Leu-Trp Medium

SD-His-Met-Ade medium

SD-His-Met-Trp Medium

SD-His-Ade-Trp medium

SD-Leu-Met-Ade Medium

SD-Leu-Met-Trp Medium

SD-Met-Ade-Trp Medium


SD four-deficient medium:

SD-Ura-His-Leu-Met medium

SD-Ura-His-Leu-Ade medium

SD-Ura-His-Leu-Trp Medium

SD-Ura-His-Met-Ade medium

SD-Ura-His-Met-Trp Medium

SD-Ura-His-Ade-Trp medium

SD-Ura-Leu-Met-Ade Medium

SD-Ura-Leu-Met-Trp medium

SD-Ura-Leu-Ade-Trp medium

SD-Ura-Met-Ade-Trp medium

SD-His-Leu-Met-Ade medium

SD-His-Leu-Met-Trp medium

SD-His-Leu-Ade-Trp medium

SD-His-Met-Ade-Trp medium

SD-Leu-Met-Ade-Trp Medium


SD Five-Deficient Medium:

SD-Ura-His-Leu-Met-Ade Medium

SD-Ura-His-Leu-Met-Trp medium

SD-Ura-His-Leu-Ade-Trp medium

SD-Ura-His-Met-Ade-Trp medium

SD-Ura-Leu-Met-Ade-Trp medium

SD-His-Leu-Met-Ade-Trp medium


SD Six Defects Medium:

SD-Ura-His-Leu-Met-Ade-Trp Medium