Yeast Synthetic Drop-out Medium, SD complete Medium
- Product Code: GMNB-SDWO00
- Availability: In Stock
Tags: SD Complete Medium
Yeast Synthetic Drop-out Medium, SD-Dropout Medium: complete medium
Packed for making 32 L of media solution:
Bottle A - Synthetic Dropout minimal base (SD): 220 g
Bottle B - Drop-Out amino acids supplement (DO): 30 g
Notice
This media is prepared in small batches and will be prepared after the purchase, taking 5 to 10 business days for handling.
Product Description:
Synthetic drop-out medium is used to screen various nutritionally deficient types of Saccharomyces cerevisiae for yeast protein expression, yeast hybrid, double-hybrid system, etc.
This product does not contain sugar. Users can add glucose, raffinose, or galactose for different experiment purposes.
Use
SD complete Medium can be used to screen for nutritionally deficient Saccharomyces cerevisiae.
SD complete Medium can be used as a protein expression medium for Saccharomyces cerevisiae when galactose is added.
SD complete Medium added with glucose can be used as a medium for the enrichment of defective yeast.
Can be used as a control to identify nutritionally deficient types of Saccharomyces cerevisiae.
Storage: in a cool and dry place.
Product properties: light yellow powder. when in the form of liquid: light amber, transparent, no precipitation or slight precipitation (insoluble calcium sulphate).
Components, per litre
Synthetic Dropout minimal base (SD):
Yeast base nitrogen source (YNB) 1.7g
Ammonium sulphate 5g
Drop-Out amino acids supplement (DO):
L-Arginine 50 mg
L-Aspartic Acid 80 mg
L-Histidine 20 mg
L-Isoleucine 50 mg
L-Leucine 100 mg
L-Lysine 50 mg
L-Methionine 20 mg
L-Phenylalanine 50 mg
L-Threonine 200 mg
L-Tryptophan 50 mg
L-Tyrosine 50 mg
L-Valine 150 mg
Adenine 40 mg
Uracil 20 mg
Preparation
[Fast preparation]
Dissolve 6.7 g of mixture A (SD), 0.93 g of mixture B (DO), and a suitable amount of agar [1] and sugar [2] in distilled or Milli-Q Water, make it to 1000ml.
PH adjustment is not usually required, but if the MQ water used is particularly acidic, adjust the pH to 5.8 before autoclaving.
Sterilise by autoclaving at 121°C for 15 minutes [3]. Aliquot as needed.
[1] 10~15g Agar for making agar plates.
[2] optionally add a suitable amount and appropriate type of carbon source.
[3] If equipment allows, autoclave at 115°C for 30 minutes to prevent the darkening of the solution due to a Maillard reaction on sugar catalysed by ammonium sulphate and heat.
[Standard preparation]
1. Prepare 0.5 litre of 10X SD stock solution:
Dissolve 33.5 g mixture A (SD) and optionally a suitable amount of sugar [1][2] in distilled or Milli-Q water and mix well, make it to 500 ml [3]. Sterilise by 0.22µm filtration (recommended) or autoclave at 115°C for 30 minutes. Store the stock at room temperature. The shelf life of this solution is approximately one year.
2. Dissolve 0.93 g of mixture B (DO), and a suitable amount of agar, in distilled or Milli-Q Water, make it to 900ml [3]. Sterilise by autoclaving at 121°C for 15 minutes.
3. Add 100 mL of sterile 10x SD stock into the freshly autoclaved (no longer boiling) mixture B (DO) solution, mix well and pour the plates while hot. Store the SD single-defective medium agar plates at 4°C. The shelf life is several months.
[1] Optionally add a suitable amount and appropriate type of carbon source. For example, add 100g dextrose for a final concentration of 20 g/L.
[2] Or add pre-prepared sugar stock, e.g. 20% w/v dextrose stock (Sterilised by 0.22µm filtration) also works.
[3] PH adjustment is not usually required, but if the MQ water used is particularly acidic, adjust the pH to 5.8 before autoclaving or filtering sterilise.
FAQ
Q1. Why do the visible colonies (approximately 1 mm in diameter) appear slower than expected?
A1: Yeast Synthetic Drop-out Medium, as a selection medium for isolating different strains and colonies on agar plates or in liquid media containing corresponding antibiotics, is designed with the intentional exclusion of carbon sources (sugars), as indicated in the product description. The standard Yeast Synthetic Drop-out Medium primarily contains essential inorganic salts (ammonium sulphate), trace minerals, vitamins such as biotin derived from YNB, and defined amino acids.
The absence of a carbon source in most selection media is intended to maintain bacterial growth in a stress state, ensuring that no excess nutrients are directed into non-essential metabolic pathways. This minimizes false positives during strain or colony selection and reduces the risk of microbial contamination during plating operations. While this approach inevitably slows bacterial growth, users may choose to supplement the media with a carbon source (e.g., glucose, raffinose, or galactose for certain Saccharomyces cerevisiae strains, or glycerol or methanol for Pichia pastoris) to enhance growth as needed. It is advised not to exceed 20 g of glucose per 6.7 g SD for general applications or 20 g of glucose per 13.4 g SD for Pichia pastoris; however, a more conservative range of 2.5–5 g of glucose per 6.7 g SD is often recommended.
Note: When using Yeast Synthetic Drop-out Medium, visible colonies (approximately 1 mm in diameter) typically emerge within 48–72 hours post-plating, which is considered normal. Growth speed is influenced by several factors, such as culturing temperature (commonly recommended at 25–30°C for Saccharomyces cerevisiae) and the strain’s genotype. In cases where genes of interest (GOI) compete for nutrients with essential metabolic pathways, the random integration of multiple GOI copies into the genome (where the copy number is typically more than one) can significantly increase cellular stress, thereby slowing growth. Additionally, strong promoters, particularly constitutive promoters for GOI, can exacerbate cellular stress as immature cells struggle to meet metabolic demands, further reducing growth rates.
Q2. What is the difference between Growth Media and Selection Media? Can Yeast Synthetic Drop-out Medium be used to culture pre-selected bacterial strains?
A2: For culturing pre-selected bacterial strains, growth media such as YPD or YPG are more appropriate than selection media. Growth media are enriched with abundant carbon sources (e.g., dextrose, glucose, glycerol, or methanol), nitrogen sources (derived from peptone), trace minerals, and vitamins (sourced from yeast extract). Many growth media also include buffering agents to maintain optimal pH levels, fostering rapid and sustained bacterial growth under ideal conditions.
Additionally, growth media are generally more cost-effective than selection media, making them an economically favourable choice for large-scale culturing.
Q3. What is the difference between Synthetic Dropout Minimal Media (SD Media) and Minimal Dextrose Medium (MD Media)?
A3: The mixture commonly known as Synthetic Dropout Minimal Base (SD) consists of Yeast Nitrogen Base (YNB, without amino acids or ammonium sulfate) and ammonium sulfate. Unlike Minimal Dextrose Medium (MD Media), SD Media lacks sugar components.
In general, SD Media is more precisely defined and is suitable for selection purposes on agar plates. Conversely, MD Media, which contains sugar, is better suited for liquid media selection when combined with antibiotics. Additionally, the growth rate in MD Media is typically faster than that in SD Media for the same strain.
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We provide a variety of customised yeast SD-Dropout media. The catalogues are listed below, and customers can request a quote and order less commonly used SD-Dropout media via support@gmexpression.com
Yeast SD Defective Medium （corresponding defective component is removed from the complete medium formulation):
SD complete medium
SD Single Defective Medium: (view product)
SD-Ura medium
SD-His medium
SD-Leu medium
SD-Met medium
SD-Ade medium
SD-Trp medium
SD Double Defective Medium: (view product)
SD-Ura-His medium
SD-Ura-Leu Medium
SD-Ura-Met medium
SD-Ura-Ade medium
SD-Ura-Trp medium
SD-His-Leu medium
SD-His-Met medium
SD-His-Ade medium
SD-His-Trp medium
SD-Leu-Met medium
SD-Leu-Ade medium
SD-Leu-Trp medium
SD-Met-Ade medium
SD-Met-Trp Medium
SD-Ade-Trp Medium
SD Triple Deletion Medium:
SD-Ura-His-Leu Medium
SD-Ura-His-Met medium
SD-Ura-His-Ade medium
SD-Ura-His-Trp Medium
SD-Ura-Leu-Met medium
SD-Ura-Leu-Ade medium
SD-Ura-Leu-Trp Medium
SD-Ura-Met-Ade medium
SD-Ura-Met-Trp Medium
SD-Ura-Ade-Trp Medium
SD-His-Leu-Met Medium
SD-His-Leu-Ade medium
SD-His-Leu-Trp Medium
SD-His-Met-Ade medium
SD-His-Met-Trp Medium
SD-His-Ade-Trp medium
SD-Leu-Met-Ade Medium
SD-Leu-Met-Trp Medium
SD-Met-Ade-Trp Medium
SD four-deficient medium:
SD-Ura-His-Leu-Met medium
SD-Ura-His-Leu-Ade medium
SD-Ura-His-Leu-Trp Medium
SD-Ura-His-Met-Ade medium
SD-Ura-His-Met-Trp Medium
SD-Ura-His-Ade-Trp medium
SD-Ura-Leu-Met-Ade Medium
SD-Ura-Leu-Met-Trp medium
SD-Ura-Leu-Ade-Trp medium
SD-Ura-Met-Ade-Trp medium
SD-His-Leu-Met-Ade medium
SD-His-Leu-Met-Trp medium
SD-His-Leu-Ade-Trp medium
SD-His-Met-Ade-Trp medium
SD-Leu-Met-Ade-Trp Medium
SD Five-Deficient Medium:
SD-Ura-His-Leu-Met-Ade Medium
SD-Ura-His-Leu-Met-Trp medium
SD-Ura-His-Leu-Ade-Trp medium
SD-Ura-His-Met-Ade-Trp medium
SD-Ura-Leu-Met-Ade-Trp medium
SD-His-Leu-Met-Ade-Trp medium
SD Six Defects Medium:
SD-Ura-His-Leu-Met-Ade-Trp Medium
