ATCC Trace Mineral & Vitamin Supplement | 250mL per bottle
- Product Code: GMNB-ATCC-TMVS
- Availability: In Stock
ATCC Trace Mineral & Vitamin Supplement, 250ml pack
Two sterile stock solutions (250 mL each):
• ATCC Vitamin Mix
• ATCC Trace Mineral Mix
Sterile‐filtered at 0.22 µm or 0.08 µm
-- 0.08 µm filter-sterile ensures complete mycoplasma removal, ideal for experimental scenarios where cultured bacteria are further in contact with mammalian cells
50X concentrated stock available. The 50X stock is optimised for precise addition, via insulin syringe, into autoclaved, anaerobic media, ideal for Hungate tube preparation. (enquiry via support@gmexpression.com)
Use
The ATCC Vitamin and Trace Mineral Mix is supplied in two 250 mL sterile stock solutions, integrating all essential vitamins and trace minerals for various ATCC-recommended media, particularly in extremophile cultivation.
This consolidated formulation streamlines media preparation and minimises handling errors.
|
Package contains |
|
|
ATCC Vitamin stock |
250 mL sterile stock |
|
ATCC Trace Mineral stock |
250 mL sterile stock |
Composition - Per 1000mL of Stock contains
|
Vitamin Stock (based on Wolfe’s solution) |
|
|
Folic acid -- (Vitamin B9) |
2 mg |
|
Pyridoxine·HCl -- (Vitamin B6) |
10 mg |
|
Riboflavin -- (Vitamin B2) |
5 mg |
|
Biotin -- (Vitamin B7) |
2 mg |
|
Thiamine·HCl -- (Vitamin B1) |
5 mg |
|
Nicotinic acid -- (Vitamin B3) |
5 mg |
|
D-Ca-pantothenate -- (Vitamin B5) |
5 mg |
|
Vitamin B12 |
0.1 mg |
|
α-Lipoic acid |
5 mg |
|
Monopotassium phosphate |
900 mg |
Trace Mineral Stock (based on Wolfe’s solution) |
|
|
EDTA |
0.50 g |
|
MgSO4 ·7H2O |
3.00 g |
|
MnSO4 ·H2O |
0.50 g |
|
NaCl |
1.00 g |
|
FeSO₄·7H₂O |
0.10 g |
|
Co(NO₃)₂·6H₂O |
0.10 g |
|
CaCl₂ |
0.10 g |
|
ZnSO₄·7H₂O |
0.10 g |
|
CuSO₄·5H₂O |
0.01 g |
|
AlK(SO₄)₂ |
0.01 g |
|
H₃BO₃ |
0.01 g |
|
Na₂MoO₄·2H₂O |
0.01 g |
|
Na₂SeO₃ |
0.001 g |
|
Na₂WO₄·2H₂O |
0.01 g |
|
NiCl₂·6H₂O |
0.02 g |
Preparation
Conduct all procedures within a clean bench or a fully sterilised anaerobic glovebox.
- Aseptically aliquot the stock solution into sterile small tubes (e.g., 10 mL conical tubes with screw cap) prior to use to prevent the stock been contamination.
- Anaerobic Supplementation
Option 1. In an anaerobic glovebox or chamber, pipette the required volume of Vitamin Mix and Trace Mineral Mix from aliquoted tubes into the target medium.
Option 2. If an anaerobic environment isn’t available, use a sterile 0.5 mL or 1 mL insulin syringe[1] to pierce the septum or rubber stopper of anaerobic bottles/Hungate tubes and inject the Vitamin & Mineral supplements.
- Gently invert or swirl to mix thoroughly, then inoculate with the bacterial sample.
- Transfer to the incubator under appropriate anaerobic conditions.
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[1] Disposable mini Insulin syringes are available from our store.
Storage
The unused stock and aliquoted tubes for multi-use are suggested to be stored at 4 °C; this best prevents the stock from becoming contaminated.
