YCFA Medium, without Vitamins & Fatty Acids | 250g
- Product Code: GMNB-YCFA01
- Availability: In Stock
Tags: YCFA Medium
YCFA Medium (Yeast Casitone Fatty Acids Broth), 250g, without Vitamins and Fatty Acids
YCFA Medium is an enriched nonselective media for cultivating most anaerobic bacteria and other fastidious microorganisms.
For a full recipe with Vitamins and Fatty Acids, please see the YCFA Medium, Full Recipe. & YCFA Modified Medium.
Use: For microbiological culture.
Per 1000mL of YCFA Medium (w.o. Vitamin and Fatty Acids) contains
|
Casein peptone |
10 g |
|
Yeast extract |
2.5 g |
|
Resazurin |
0.001 g |
|
Dipotassium hydrogen phosphate |
0.45 g |
|
Potassium dihydrogen phosphate |
0.45 g |
|
Sodium chloride |
0.9 g |
|
Sodium bicarbonate |
4 g |
|
Magnesium sulphate |
0.044 g |
|
Calcium chloride |
0.09 g |
Preparation
Weigh 18.44g of this product, heat and dissolve in 1000ml distilled water, 121℃ autoclave for 15 minutes.
Note:
1. It's a common option to add an appropriate amount of sugar (glucose, maltose and cellobiose each with a final concentration of 2.0 g/L; or only glucose with a final concentration of 5.0 g/L), amino acid (L-cysteine HCl 1.0g/L), vitamins and volatile fatty acids according to the use.
2. Add an appropriate amount of agar (1~2% w/w) to prepare a semi-solid or solid culture medium.
Caution:
When YCFA Medium is prepared, the solution may quickly produce a lot of foam (carbon dioxide produced by sodium bicarbonate when it meets acidic liquid), which is normal and the foam will disappear after a few minutes of resting.
