Sheep Blood Agar Base, with defibrinated Blood | 250g

Sheep Blood Agar Base | with defibrinated Blood

-  Sheep Blood Agar Base 250g   +   Defibrinated Blood 500ml  (optional)

Sheep Blood Agar is an enriched medium provided with multiple nutrients that generally comes as a basal media for the preparation of blood agar by supplementation with blood.

Cautions

·  Laboratory procedures involving pathogens require special equipment and techniques to minimise biohazards.

Per 1000mL of Sheep Blood Agar Base contains

*  Adjusted and/or supplemented as required to meet performance criteria.

*  Blood products have a maximum shelf life of 35 days, and the Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Please consider the requirements before purchasing.

Preparation

-       Dissolve 31.0 g of mixture A in 900~950 ml of MQ water in a closed container. 

-       Autoclave at 121°C for 15 minutes.

-       Cool the Base: Cool the sterile molten agar base to 45–50°C, typically in a water bath to maintain a consistent temperature and prevent premature solidification.

-       Add Blood: Aseptically add 5-10% (v/v, final) sterile defibrinated sheep blood that has been pre-warmed to room temperature.

-       Mix and Pour: Mix gently by swirling the flask or bottle, avoiding air bubbles. Quickly dispense the mixture into sterile Petri dishes.

-       Solidify and Store: Allow the plates to solidify completely at room temperature (around 30 minutes to an hour) before inverting and storing them in sealed plastic bags at 2–8°C to prevent drying and condensation.

Note:

It is strongly recommended to operate within a clean bench when preparing the solution & agar plate.

When ordered with defibrinated blood, this item is shipped with 2.8 kg of dry ice for international deliveries and 1.3 kg of ice packs for domestic shipments.

For international customers, we recommend sourcing defibrinated sheep or horse blood locally whenever possible to minimise the risk of customs clearance delays, which are common when importing animal blood products.