YNB media, Yeast Nitrogen Base, without Amino Acids | 20X Stock (13.4% w/w) | 500mL, specific for Pichia pastoris

YNB without Amino Acids

500 ml Liquid | 20X Stock (13.4% w/w) 

This product equals 10X YNB Stock according to the Pichia culture standard with doubled components concentration.

0.22μm filtration plus 5min 275/365nm UV treatment sterilised, Suggest storage in 4°C / 39.2 ℉ refrigerator.

Applications

For Saccharomyces cerevisiae, Pichia pastoris culturing

Components, mg per litre

Yeast Nitrogen Base (YNB), per litre

Preparation

Dissolve 1 or 2 volumes of YNB 20X stock in 16~18 volumes of million-Q water, with a suitable amount of Dextrose stock, and amino acids stock, make up to the final volume and use directly.

Minimal Dextrose Medium (MD media) for Saccharomyces cerevisiae

0.67% YNB

5% Dextrose (by adding 5X D stock | 25% D-glucose)

Minimal Dextrose Medium (MD media) for Pichia pastoris

1.34% YNB

0.00004% Biotin (by adding 500X B stock | 0.02% Biotin)

2% Dextrose (by adding 10X D stock | 20% D-glucose)

Minimal Methanol Medium（MM media) for Pichia pastoris

1.34% YNB

0.00004% Biotin (by adding 500X B stock | 0.02% Biotin)

0.5% Methanol (by adding 10X M stock | 5% Methanol)

Minimal Glycerol Medium（MG media) for Pichia pastoris

1.34% YNB

0.00004% Biotin (by adding 500X B stock | 0.02% Biotin)

1% Glycerol (by adding 10X GY stock | 10% Glycerol)

For plates

Add 15 g agar to 800 mL of MQ water and autoclave for 20 minutes on the liquid cycle. Cool to about 60°C and then add sterile YNB/B/D/GY stock for a specific medium, make the final volume and pour the plates immediately.

After opening, the remaining liquid should be sealed immediately and stored in the 4°C refrigerator. Operation on a sterile bench is recommended.