YNB media, Yeast Nitrogen Base, without Amino Acids | 20X Stock (13.4% w/w) | 500mL, specific for Pichia pastoris
- Product Code: GMNB-YNB120L
- Availability: In Stock
Tags: Liquid YNB media
YNB
without Amino Acids
500 ml Liquid | 20X Stock (13.4% w/w)
This product equals 10X YNB Stock according to the Pichia culture standard with doubled components concentration.
0.22μm filtration plus 5min 275/365nm UV treatment sterilised,
Suggest storage in 4°C / 39.2 ℉ refrigerator.
Applications
For Saccharomyces cerevisiae, Pichia pastoris culturing
Components,
mg per litre
Yeast Nitrogen Base (YNB), per litre
Ammonium sulfate |
5.0 g |
Biotin |
2 μg |
Calcium pantothenate |
400 μg |
Folic acid |
2 μg |
Inositol |
2000 μg |
Niacin |
400 μg |
p-Aminobenzoic acid |
200 μg |
Pyridoxine hydrochloride |
400 μg |
Riboflavin |
200 μg |
Thiamine hydrochloride |
400 μg |
Boric acid |
500 μg |
Copper sulfate |
40 μg |
Potassium iodide |
100 μg |
Ferric chloride |
200 μg |
Manganese sulfate |
400 μg |
Sodium molybdate |
200 μg |
Zinc sulfate |
400 μg |
Potassium phosphate monobasic |
1 g |
Magnesium sulfate |
500 mg |
Sodium chloride |
100 mg |
Calcium chloride |
100 mg |
Preparation
Dissolve 1 or 2 volumes of YNB 20X stock in 16~18 volumes of million-Q water, with a suitable amount of Dextrose stock, and
amino acids stock, make up to the final volume and use directly.
Minimal Dextrose Medium (MD media) for Saccharomyces cerevisiae
0.67% YNB
5% Dextrose (by adding 5X D stock |
25% D-glucose)
Minimal Dextrose Medium (MD media) for Pichia pastoris
1.34% YNB
0.00004% Biotin (by adding 500X B
stock | 0.02% Biotin)
2% Dextrose (by adding 10X D stock |
20% D-glucose)
Minimal Methanol Medium(MM media) for Pichia
pastoris
1.34% YNB
0.00004% Biotin (by adding 500X B
stock | 0.02% Biotin)
0.5% Methanol (by adding 10X M stock
| 5% Methanol)
Minimal Glycerol Medium(MG media) for Pichia
pastoris
1.34% YNB
0.00004% Biotin (by adding 500X B
stock | 0.02% Biotin)
1% Glycerol (by adding 10X GY stock |
10% Glycerol)
For plates
Add 15 g agar to 800 mL of MQ water
and autoclave for 20 minutes on the liquid cycle. Cool to about 60°C and then
add sterile YNB/B/D/GY stock for a specific medium, make the final volume and
pour the plates immediately.
After opening, the remaining liquid
should be sealed immediately and stored in the 4°C refrigerator. Operation on a
sterile bench is recommended.