• YNB media, Yeast Nitrogen Base, without Amino Acids | 20X Stock (13.4% w/w) | 500mL, specific for Pichia pastoris

YNB media, Yeast Nitrogen Base, without Amino Acids | 20X Stock (13.4% w/w) | 500mL, specific for Pichia pastoris

AUD 170.00    Excl.GST
  • Product Code: GMNB-YNB120L
  • Availability: In Stock

Tags: Liquid YNB media

YNB without Amino Acids

500 ml Liquid | 20X Stock (13.4% w/w) 

This product equals 10X YNB Stock according to the Pichia culture standard with doubled components concentration.


0.22μm filtration plus 5min 275/365nm UV treatment sterilised, Suggest storage in 4°C / 39.2 ℉ refrigerator.


Applications

For Saccharomyces cerevisiae, Pichia pastoris culturing

Components, mg per litre

Yeast Nitrogen Base (YNB), per litre

Ammonium sulfate

5.0 g

Biotin 

2 μg

Calcium pantothenate 

400 μg

Folic acid

2 μg

Inositol 

2000 μg

Niacin 

400 μg

p-Aminobenzoic acid

200 μg

Pyridoxine hydrochloride

400 μg

Riboflavin 

200 μg

Thiamine hydrochloride

400 μg

Boric acid

500 μg

Copper sulfate

40 μg

Potassium iodide

100 μg

Ferric chloride

200 μg

Manganese sulfate

400 μg

Sodium molybdate 

200 μg

Zinc sulfate

400 μg

Potassium phosphate monobasic 

1 g

Magnesium sulfate

500 mg

Sodium chloride

100 mg

Calcium chloride

100 mg

 

Preparation

Dissolve 1 or 2 volumes of YNB 20X stock in 16~18 volumes of million-Q water, with a suitable amount of Dextrose stock, and amino acids stock, make up to the final volume and use directly.

Minimal Dextrose Medium (MD media) for Saccharomyces cerevisiae

0.67% YNB

5% Dextrose (by adding 5X D stock | 25% D-glucose)

Minimal Dextrose Medium (MD media) for Pichia pastoris

1.34% YNB

0.00004% Biotin (by adding 500X B stock | 0.02% Biotin)

2% Dextrose (by adding 10X D stock | 20% D-glucose)

Minimal Methanol MediumMM media) for Pichia pastoris

1.34% YNB

0.00004% Biotin (by adding 500X B stock | 0.02% Biotin)

0.5% Methanol (by adding 10X M stock | 5% Methanol)

Minimal Glycerol MediumMG media) for Pichia pastoris

1.34% YNB

0.00004% Biotin (by adding 500X B stock | 0.02% Biotin)

1% Glycerol (by adding 10X GY stock | 10% Glycerol)

For plates

Add 15 g agar to 800 mL of MQ water and autoclave for 20 minutes on the liquid cycle. Cool to about 60°C and then add sterile YNB/B/D/GY stock for a specific medium, make the final volume and pour the plates immediately.

After opening, the remaining liquid should be sealed immediately and stored in the 4°C refrigerator. Operation on a sterile bench is recommended.

 

Safety Data Sheet:

SDS_en


Certificate of Analysis - 2024

COA-GMNB-YNB120L-20240105