Nitrogen-free Medium | 250g
AUD 90.00
Excl.GST
- Product Code: GMNB-NFM01
- Availability: In Stock
Tags: Nitrogen-free Medium
Nitrogen-free Medium | 250g
Use
For the cultivation of autochthonous nitrogen-fixing bacteria and potassium bacteria
Per 1000mL of Nitrogen-free Medium contains
|
Mannitol |
10.0 g |
|
MgSO4 ·7H2O |
0.2 g |
|
CaCl2 · 2H2O |
0.2 g |
|
CaCO3
|
5.0 g |
|
KH2PO4 |
0.2 g |
|
NaCl |
0.2 g |
|
Agar |
20.0 g |
Preparation
- Dissolve 35.8 g of media in 900 ml of MQ water， and make it to 1000 ml with MQ water. Adjust the pH to 7.0-7.2 using 1M KOH or 1M HCl.
- Autoclave at 115°C for 30 minutes.
- After autoclaving, calcium carbonate will precipitate at the bottom of the solution, It is recommended to shake well at 65~70°C just before pouring agar plates.
