Hygromycin B | 200mg

Hygromycin B

Hygromycin B HCl salt

Other names: Hygromix;  O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine

CAS Number:  31282-04-9

Molecular formula:  C20H37N3O13 HCL

Storage：2-8°C refrigeration

Molecular weight: 527.53 g/mol

Grade: USP grade

Potency: ≥900mg/g

Moisture: ≤5.0%

Properties:

white or brown-white powder.

solubility H2O: 50 mg/mL

Uses

For biochemical research. Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

In the laboratory, Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation.[4] Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells.

Low toxicity to plant cells, Plant cell toxicity threshold ~200μg/ml

Recommended Stock Concentration        50 mg/mL

Working concentration : 

Plant histoculture  ~  100 μg/ml;

Cell selection ~   50-800 μg/mL, specifically 50~100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Cautions 

Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.

This product is restricted to scientific research only and should not be used for clinical therapy or other purposes.