Acetate Buffer, pH 5.0 | Sodium Acetate, pH 5.2 | 500ml
AUD 39.00
Excl.GST
- Product Code: GMES-B-G8
- Availability: In Stock
Tags: Buffer
Acetate buffering is the standard simple acid-range system for pH 4.5-5.5 work. The GMExpression's Acetate Buffer line is best launched as two closely related SKUs: a 1 M pH 5.0 chromatography buffer and a 3 M pH 5.2 sodium acetate stock for nucleic-acid precipitation.
Overview
Acetate Buffer, pH 5.0 / Sodium Acetate, pH 5.2 - Acetate buffering is the standard simple acid-range system for pH 4.5-5.5 work. The GMExpression's Acetate Buffer line is best launched as two closely related SKUs: a 1 M pH 5.0 chromatography buffer and a 3 M pH 5.2 sodium acetate stock for nucleic-acid precipitation.
This page positions both variants together because laboratories often need one or both in RNA, DNA, and acidic protein-biochemistry workflows.
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Use and Applications
- RNA and DNA precipitation using sodium acetate and ethanol
- Cation-exchange chromatography of basic proteins
- Glycoprotein and acidic hydrolase assay buffers
- General pH 5.0 biochemical buffering
Specifications
| Item | Specification |
|---|---|
| Acetate Buffer | 1 M, pH 5.0, 500 mL |
| Sodium Acetate | 3 M, pH 5.2, 500 mL |
| Sterility | 0.22 μm sterile-filtered |
Composition
| Component | Amount / concentration | Function |
|---|---|---|
| Sodium acetate | 1 M or 3 M; CAS 6131-90-4 / 127-09-3 | Acetate salt component |
| Acetic acid | q.s.; CAS 64-19-7 | Acid component for pH adjustment |
| NaOH | minor trim if required | Final pH adjustment |
| Milli-Q water | q.s. to final volume | Solvent |
Instruction
1For nucleic-acid precipitation, add 0.1 volume of 3 M sodium acetate pH 5.2 to the sample, then add ethanol or isopropanol according to the protocol.
2For chromatography or assay buffers, dilute the 1 M pH 5.0 stock to the desired final concentration.
3Verify pH after adding high salt, detergent, or protein stabilisers.
Note
- The 3 M sodium acetate variant is intentionally concentrated for precipitation workflows, not for direct assay use.
Cautions
Caution. Acetic acid is volatile and irritating; keep containers tightly capped.
Caution. High acetate concentrations can interfere with some downstream enzymatic reactions if not removed by washing or dilution.
Storage and Expiry · Safety
- Store at room temperature or 2-8 deg C.
- Unopened shelf life: 24 months from manufacture after QC validation.
- Transport. Ships at ambient temperature; no cold chain required. Protect from freezing and from temperatures above 40 deg C.
- Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. Contains acetic acid, which is volatile and irritating to eyes and the respiratory tract; handle in a well-ventilated area. Consult the Safety Data Sheet before use.
References
- Sambrook and Russell, Molecular Cloning, 3rd edition.
- Safety Data Sheet.
Frequently Asked Questions
Q1. Why include two sodium acetate variants?
They serve different workflows: 1 M pH 5.0 for buffer formulation, 3 M pH 5.2 for nucleic-acid precipitation.
Q2. Can this be supplied RNase-free?
Yes. The sodium acetate precipitation variant can be prepared with RNase-free water and packaging. Quotation separately via support@gmexpression.com
