Columbia Agar Base 220g | with defibrinated Blood

Columbia Agar Base | with defibrinated Blood

-  Columbia Agar Base 220g   +   Defibrinated Blood 500ml  (optional)

Use

Columbia Agar Base is a nutrient-rich medium for the cultivation of fastidious organisms, commonly used as a base for Blood Chocolate Agar. It can also be used as a selective isolation medium by the addition of antimicrobial agents, suitable for the isolation and cultivation of a wide variety of microorganisms that are difficult to grow, like Neisseria meningitidis,Neisseria gonorrhoeae and Haemophilus influenzae. By adding blood, it can be used to determine haemolytic reactions, while the majority of common pathogenic bacteria grow well without the addition of blood. 

Pancreatic digest provides general nitrogen, vitamins, minerals and amino acids, while yeast extract and maize starch are a source of vitamins essential for bacterial growth. Sodium chloride provides essential electrolytes for transport and osmotic balance. Blood is another source of growth factors for microorganisms and is the basis for determining haemolytic reactions.

Cautions

·  Laboratory procedures involving pathogens require special equipment and techniques to minimise biohazards.

Per 1000mL of Columbia Agar Base contains

*  Adjusted and/or supplemented as required to meet performance criteria.

*  Blood products have a maximum shelf life of 35 days， and the Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Please consider the requirements before purchasing.

**  Haemolytic reactions are unavailable if using Blood Alternative to prepare the media.

Preparation

-       Dissolve 44 g of mixture A in 900~950 ml of MQ water in a closed container.

-       Autoclave at 121°C for 15 minutes.

-      Cool the broth stock to about 70°C. Aseptically add 5~10% (w/w, final) sterile defibrinated blood, keep at 70°C for 15 minutes and cool to about 50°C. Mix and quickly dispense into sterile petri dishes.

Note:

It is strongly recommended to operate within a clean bench when preparing the solution & agar plate.

When ordering with the blood stock, this item will be packaged with 2.8kg of dry ice as standard for international shipping. For domestic shipping, it is packaged with a 1.3kg ice bag.

International buyers are suggested to purchase defibrinated sheep blood or horse blood locally as far as possible, unless it is difficult to do so, to avoid risks in customs clearance at the point of entry, which is common for importing animal blood products.