Overview

SSC Buffer, 20x Stock, pH 7.0 - SSC Buffer is the standard high-salt citrate buffer base for nucleic-acid hybridisation and wash stringency control. A 20x stock lets users prepare 0.1x-6x working solutions depending on probe, target, and temperature conditions.

This candidate is a workflow-extension SKU for customers working in FISH, RNA-FISH, Southern blot, Northern blot, and hybridisation-chain-reaction protocols.

We also have

S30A Buffer · S30B Buffer · GMExpression Buffer catalogue 

Use and Applications

  • Southern and Northern blot hybridisation and washing
  • FISH and RNA-FISH probe hybridisation
  • Hybridisation Chain Reaction (HCR) workflows
  • Microbiome localisation and tissue-section nucleic-acid detection

→Buffer Indicator Navigator

Use the GMExpression Buffer Indicator Navigator to find suitable pH ranges that match this buffer system, or start from a specific buffer and search for suitable working pH ranges for the intended experiment.

Specifications

ItemSpecification
Bottle500 mL bottle
Concentration20x stock
Composition3 M NaCl, 0.3 M trisodium citrate, pH 7.0
Sterility0.2 um filtered

Composition

ComponentAmount / concentrationFunction
Sodium chloride3 M; CAS 7647-14-5Hybridisation ionic strength
Trisodium citrate dihydrate0.3 M; CAS 6132-04-3Citrate buffer and chelator
HClq.s. to pH 7.0pH adjustment
Ultrapure waterq.s. to final volumeSolvent

Instruction

1Dilute the 20x stock with ultrapure water to the protocol-specified working strength.
2Typical wash strengths range from 0.1x for high stringency to 6x for low stringency.
3Warm and mix if salt crystallisation is observed after cold storage.

Note

  • Hybridisation stringency is controlled by SSC concentration, formamide content, temperature, and probe length. Do not change SSC concentration without considering the whole protocol.

Cautions

Caution. High-salt solutions can crystallise during cold storage; fully redissolve before use.
Caution. Citrate chelates divalent cations and may interfere with metal-dependent downstream steps.

Storage and Expiry · Safety

  • Store at room temperature.
  • Unopened shelf life: 24 months from manufacture after QC validation.
  • Transport. Ships at ambient temperature; no cold chain required. Protect from freezing and from temperatures above 40 deg C.
  • Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. The formulated buffer presents no significant hazard under normal use; avoid ingestion and eye contact. Consult the Safety Data Sheet before use.

References

  1. Sambrook and Russell, Molecular Cloning, 3rd edition.
  2. GMExpression Buffer Product Line - Strategic Expansion Analysis Report, G-9.
  3. Safety Data Sheet: SDS draft to be finalised before commercial release.

Frequently Asked Questions

Q1. What working concentration should I use?
Use the concentration specified in the hybridisation protocol; SSC strength directly changes stringency.
Q2. Can SSC replace PBS?
No. SSC is a nucleic-acid hybridisation buffer, not a physiological wash buffer.