Overview

Acetate Buffer, pH 5.0 / Sodium Acetate, pH 5.2 - Acetate buffering is the standard simple acid-range system for pH 4.5-5.5 work. The GMExpression candidate is best launched as two closely related SKUs: a 1 M pH 5.0 chromatography buffer and a 3 M pH 5.2 sodium acetate stock for nucleic-acid precipitation.

This page positions both variants together because laboratories often need one or both in RNA, DNA, and acidic protein-biochemistry workflows.

We also have

S30A Buffer · S30B Buffer · GMExpression Buffer catalogue · YCFA Modified Medium · Modified Chopped Meat Broth

Use and Applications

  • RNA and DNA precipitation using sodium acetate and ethanol
  • Cation-exchange chromatography of basic proteins
  • Glycoprotein and acidic hydrolase assay buffers
  • General pH 5.0 biochemical buffering

→Buffer Indicator Navigator

Use the GMExpression Buffer Indicator Navigator to find suitable pH ranges that match this buffer system, or start from a specific buffer and search for suitable working pH ranges for the intended experiment.

Specifications

ItemSpecification
Variant AAcetate Buffer 1 M, pH 5.0, 500 mL
Variant BSodium Acetate 3 M, pH 5.2, 500 mL
Sterility0.2 um sterile-filtered

Composition - draft formulation / release specification

ComponentAmount / concentrationFunction
Sodium acetate1 M or 3 M; CAS 6131-90-4 / 127-09-3Acetate salt component
Acetic acidq.s.; CAS 64-19-7Acid component for pH adjustment
NaOHminor trim if requiredFinal pH adjustment
Ultrapure waterq.s. to final volumeSolvent

Instruction

1For nucleic-acid precipitation, add 0.1 volume of 3 M sodium acetate pH 5.2 to the sample, then add ethanol or isopropanol according to the protocol.
2For chromatography or assay buffers, dilute the 1 M pH 5.0 stock to the desired final concentration.
3Verify pH after adding high salt, detergent, or protein stabilisers.

Note

  • The 3 M sodium acetate variant is intentionally concentrated for precipitation workflows, not for direct assay use.

Cautions

Caution. Acetic acid is volatile and irritating; keep containers tightly capped.
Caution. High acetate concentrations can interfere with some downstream enzymatic reactions if not removed by washing or dilution.

Storage and Expiry · Safety

  • Store at room temperature or 2-8 deg C.
  • Unopened shelf life: 24 months from manufacture after QC validation.
  • Transport. Ships at ambient temperature; no cold chain required. Protect from freezing and from temperatures above 40 deg C.
  • Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. Contains acetic acid, which is volatile and irritating to eyes and the respiratory tract; handle in a well-ventilated area. Consult the Safety Data Sheet before use.

References

  1. GMExpression Buffer Product Line - Strategic Expansion Analysis Report, G-8.
  2. Sambrook and Russell, Molecular Cloning, 3rd edition.
  3. Safety Data Sheet: SDS draft to be finalised before commercial release.

Frequently Asked Questions

Q1. Why include two acetate variants?
They serve different workflows: 1 M pH 5.0 for buffer formulation, 3 M pH 5.2 for nucleic-acid precipitation.
Q2. Can this be supplied RNase-free?
Yes. The sodium acetate precipitation variant can be prepared with RNase-controlled water and packaging.