Reinforced Clostridial Medium (RCM)
- Product Code: GMNB-RCM01
- Availability: In Stock
Reinforced Clostridial Medium (RCM), 250g, 500g
Reinforced Clostridial Medium (RCM) is a non-selective, nutrient-rich medium formulated for the cultivation and enumeration of Clostridium spp. and other obligate anaerobes. It is widely used in food and clinical microbiology, as well as in the microbiological examination of non-sterile products in accordance with the US and European Pharmacopoeia.
For the selective cultivation of Clostridia, the Differential Reinforced Clostridial Broth (DRCM) is recommended.
Broad Anaerobic Support
Promotes the growth of a wide range of anaerobes, including Clostridium spp.
Nutrient-Dense
Contains peptone, beef extract, and yeast extract to supply essential nitrogen, amino acids, vitamins, and carbon sources.
Fermentable Carbohydrate
Glucose (dextrose) supports bacterial metabolism.
Reducing Agent
Cysteine HCl lowers redox potential to maintain anaerobic conditions.
pH Stability
Sodium acetate acts as a buffering agent.
Osmotic Balance
Sodium chloride maintains appropriate osmotic pressure.
Semisolid Format
Low agar content facilitates anaerobic growth and motility studies.
Applications
Enumeration & Cultivation
For isolating and quantifying anaerobes from food, clinical, and environmental samples.
Food Microbiology
Detection of Clostridium spp. in dairy, canned, and processed foods.
Clinical Diagnostics
Isolation of anaerobes from clinical specimens.
Pharmacopoeial Testing
Compliant with USP and EP standards for non-sterile product testing.
Component, g/L
|
Yeast extract |
13.0 g |
|
Tryptone |
10.0 g |
|
Soluble starch |
1.0 g |
|
Glucose |
5.0 g |
|
Cysteine hydrochloride |
0.5 g |
|
Sodium chloride |
5.0 g |
|
Sodium acetate |
3.0 g |
RCM may be supplemented with polymyxin B for selective applications. Visit the product page of Polymyxin B Sulphate if needed.
* adjusted PH 6.6~7 as required
Preparation
Suspend 37.5 g of medium in 1 L of distilled or Milli-Q water.
Heat till boiling with agitation until fully dissolved. Adjust PH to 6.6~7 as required.
Mix thoroughly and dispense 7 mL into Hungate tubes or 1/5–1/4 volume of anaerobic culture bottles under an anaerobic gas atmosphere (e.g., N₂-80%/H₂-10%/CO₂-10%, or N₂-100%).
Autoclave at 121 °C for 15 minutes.
For a solid medium, add 15.0g Agar. For a semi-solid medium, add 0.5g Agar to make 1000 mL of media.
