• Cetrimide Agar, with Glycerine | 250g

Cetrimide Agar, with Glycerine | 250g

AUD 195.00    Excl.GST
  • Product Code: GMNB-PA01
  • Availability: In Stock

Tags: Cetrimide Agar

Cetrimide Agar, with Glycerine | 250g


Cetrimide Agar is a selective and differential medium used for the isolation and identification of Pseudomonas aeruginosa from clinical and non-clinical specimens.  Cetrimide is the selective agent and inhibits most bacteria by acting as a detergent ( Cetyltrimethylammonium bromide, a quaternary ammonium, cationic detergent).


It is also known as Pseudomonas Cetrimide Agar or Pseudosel Agar..


Applications

Cetrimide Agar is:

  • primarily used for the selective isolation and presumptive identification of Pseudomonas aeruginosa from clinical and nonclinical specimens.
  • also used for determining the ability of an organism to produce fluorescein and pyocyanin (Antibiotica).


Components

per 1000 ml

Pancreatic Digest of Gelatin

20.0 g

Potassium Sulfate

10.0 g

Magnesium Chloride

1.4 g

Cetyltrimethylammonium Bromide

0.3 g

Agar

13.6 g

Glycerine

10 ml



Preparation

  1. Dissolve 45.3 g of Mixture A in 1000 ml of DI or MQ water.
  2. Add 10ml of glycerol and boil to dissolve completely.
  3. Autoclave at 121 °C for 15 min.
  4. Cool the medium to approximately 50°C and pour into sterile Petri dishes.